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Literature summary for 2.7.7.19 extracted from
- Purification of terminal riboadenylate transferase from calf thymus gland (1975), J. Biol. Chem., 250, 4486-4496.
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing accelerates inactivation | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | - |
Bos taurus |  |
| additional information | insensitive to high levels of RNA-polymerase inhibitors | Bos taurus | |
| Na2HPO4 | - |
Bos taurus | |
| Ribonucleoside triphosphates other than ATP | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
ATP | oligoadenylate (in presence of Mn2+) | Bos taurus | |
| 0.2 | - |
oligoadenylate | in presence of Mg2+, pH 9.0, 35°C | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Bos taurus | 5737 | - |
| soluble | - |
Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
sucrose density gradient sedimentation, gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| calf | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.33 | - |
35°C, pH 9.0 | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, 80% inactivation in the first few weeks, the 20% remaining activity is stable for more than 2 years | Bos taurus |
| -70°C or under liquid nitrogen, extensive inactivation | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + oligoadenylate | - |
Bos taurus | ? | - |
? | |
| ATP + RNA | highly specific for ATP | Bos taurus | diphosphate + RNA(A)n | - |
? | |
| ATP + RNA | primer required | Bos taurus | diphosphate + RNA(A)n | - |
? | |
| ATP + RNA | primer: variety of oligoribonucleotides having free 3'-OH | Bos taurus | diphosphate + RNA(A)n | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 62000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | - |
ATP | cosubstrates rA(pA)5, pH 9.0, 35°C | Bos taurus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.8 | about 50% of maximum activity at pH 7.0 and pH 8.8 | Bos taurus |
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Release 2023.1 (January 2023)
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