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Literature summary for 2.7.7.13 extracted from

  • Sousa, S.A.; Moreira, L.M.; Leitao, J.H.
    Functional analysis of the Burkholderia cenocepacia J2315 BceAJ protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities (2008), Appl. Microbiol. Biotechnol., 80, 1015-1022.
    View publication on PubMed
Search for more literature for 2.7.7.13

Application

Application Comment Organism
drug development the enzyme is a potential target for antimicrobial drug design, i.e. in the treatment of cystic fibrosis caused by Burkholderia cenocepacia Burkholderia cenocepacia

Cloned(Commentary)

Cloned (Comment) Organism
gene bceA, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Burkholderia cenocepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 GDP-mannose pH 7.6, recombinant enzyme, in presence of 5 mM Mg2+ and 2 mM diphosphate Burkholderia cenocepacia

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ GMP activity is strongly dependent on the presence of Ca2+ or Mn2+ Burkholderia cenocepacia
Co2+ activates slightly Burkholderia cenocepacia
Mg2+ activates Burkholderia cenocepacia
Mn2+ GMP activity is strongly dependent on the presence of Ca2+ or Mn2+, the presence of 5 mM Mn2+, precipitation occurred in the reaction mixture Burkholderia cenocepacia
additional information descending order of activation Mg2+, Ca2+, Mn2+, Co2+, Ni2+ Burkholderia cenocepacia
Ni2+ activates slightly Burkholderia cenocepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55716
-
 x * 56000, recombinant enzyme, SDS-PAGE, x * 55716, sequence caculation Burkholderia cenocepacia
56000
-
 x * 56000, recombinant enzyme, SDS-PAGE, x * 55716, sequence caculation Burkholderia cenocepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + GDP-mannose Burkholderia cenocepacia
-
 GTP + alpha-D-mannose 1-phosphate
-
 ?
additional information Burkholderia cenocepacia the enzyme of Burkholderia cenocepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities ?
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia cenocepacia B4EMQ6 gene bceA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity Burkholderia cenocepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + GDP-mannose
-
 Burkholderia cenocepacia GTP + alpha-D-mannose 1-phosphate
-
 ?
additional information the enzyme of Burkholderia cenocepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities Burkholderia cenocepacia ?
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, recombinant enzyme, SDS-PAGE, x * 55716, sequence caculation Burkholderia cenocepacia
More the enzyme's amino acid sequence contains several conserved motifs: 1. the pyrophosphorylase signature sequence 38GGXGXRLXPLX5PK54, 2. the GMP active site 210FVEKP214, 3. the zinc-binding motif 436QXH438 and the putative PMI active site 465EN(Q/E)SX (Y/F)I471 Burkholderia cenocepacia

Synonyms

Synonyms Comment Organism
BceA(J) protein
-
 Burkholderia cenocepacia
BceAJ
-
 Burkholderia cenocepacia
GDP-D-mannose pyrophosphorylase
-
 Burkholderia cenocepacia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Burkholderia cenocepacia