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Literature summary for 2.7.7.13 extracted from
- Sugar nucleotide reactions in Arthrobacter. I. Guanosine diphosphate mannose pyrophosphorylase: purification and properties (1964), J. Biol. Chem., 239, 3119-3126.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
alpha-D-mannose 1-phosphate | pH 8.2, 37°C | Arthrobacter sp. |  |
| 0.052 | - |
GDP-mannose | pH 8.2, 37°C | Arthrobacter sp. | |
| 0.09 | - |
diphosphate | pH 8.2, 37°C | Arthrobacter sp. | |
| 0.12 | - |
dGDP-mannose | pH 8.2, 37°C | Arthrobacter sp. | |
| 0.13 | - |
GTP | pH 8.2, 37°C | Arthrobacter sp. | |
| 0.28 | - |
ITP | pH 8.2, 37°C | Arthrobacter sp. | |
| 1.9 | - |
Mg2+ | pH 8.2, 37°C | Arthrobacter sp. | |
| 3.3 | - |
IDP-mannose | pH 8.2, 37°C | Arthrobacter sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + alpha-D-mannose 1-phosphate | Arthrobacter sp. | - |
GDPmannose + diphosphate | - |
r | |
| GTP + alpha-D-mannose 1-phosphate | Arthrobacter sp. NRRL B1973 | - |
GDPmannose + diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter sp. | - |
NRRL B1973 | - |
| Arthrobacter sp. NRRL B1973 | - |
NRRL B1973 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protamine sulfate, Sephadex G-25, DEAE-cellulose | Arthrobacter sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.4 | - |
- |
Arthrobacter sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dGTP + alpha-D-mannose 1-phosphate | 8% of activity with GDP-mannose | Arthrobacter sp. | dGDPmannose + diphosphate | - |
r | |
| dGTP + alpha-D-mannose 1-phosphate | 8% of activity with GDP-mannose | Arthrobacter sp. NRRL B1973 | dGDPmannose + diphosphate | - |
r | |
| GTP + alpha-D-mannose 1-phosphate | - |
Arthrobacter sp. | GDPmannose + diphosphate | - |
r | |
| GTP + alpha-D-mannose 1-phosphate | equilibrium constant: 2.5 | Arthrobacter sp. | GDPmannose + diphosphate | - |
r | |
| GTP + alpha-D-mannose 1-phosphate | - |
Arthrobacter sp. NRRL B1973 | GDPmannose + diphosphate | - |
r | |
| GTP + alpha-D-mannose 1-phosphate | equilibrium constant: 2.5 | Arthrobacter sp. NRRL B1973 | GDPmannose + diphosphate | - |
r | |
| GTP + alpha-D-mannose 1-phosphate | - |
Arthrobacter sp. | diphosphate + GDP-mannose | - |
? | |
| GTP + alpha-D-mannose 1-phosphate | - |
Arthrobacter sp. NRRL B1973 | diphosphate + GDP-mannose | - |
? | |
| ITP + alpha-D-mannose 1-phosphate | 11% of activity with GDP-mannose | Arthrobacter sp. | IDPmannose + diphosphate | - |
r | |
| ITP + alpha-D-mannose 1-phosphate | 11% of activity with GDP-mannose | Arthrobacter sp. NRRL B1973 | IDPmannose + diphosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Arthrobacter sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48 | - |
30 min, 50% loss of activity | Arthrobacter sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Arthrobacter sp. |
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