Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Salmonella enterica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP-mannose | competitive vs. GTP, uncompetitive vs. mannose 1-phosphate | Salmonella enterica | |
GDP-mannose | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
alpha-D-mannose 1-phosphate | - |
Salmonella enterica | |
0.2 | - |
GTP | - |
Salmonella enterica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54045 | - |
2 * 54045, recombinant enzyme, deduced from nucleotide sequence | Salmonella enterica |
55000 | - |
2 * 55000, recombinant enzyme, SDS-PAGE | Salmonella enterica |
108000 | - |
recombinant enzyme, gel filtration | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | Sus scrofa | - |
GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | Arthrobacter sp. | - |
GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | Salmonella enterica | - |
GDPmannose + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
- |
- |
Salmonella enterica | - |
group B | - |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, Q-Sepharose FF, Phenyl-Sepharose, diafiltration, Sephadex G 75, Sephadex G 200, ammonium sulfate | Salmonella enterica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.75 | - |
recombinant enzyme | Salmonella enterica |
Storage Stability | Organism |
---|---|
4°C, 25% glycerol, 47 d, 25% loss of activity | Salmonella enterica |
4°C, 3 M ammonium sulfate, 47 d, 40% loss of activity, 122 d, 50% loss of activity | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | - |
Sus scrofa | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | - |
Arthrobacter sp. | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | - |
Salmonella enterica | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | - |
Salmonella enterica | diphosphate + GDP-mannose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, recombinant enzyme, SDS-PAGE | Salmonella enterica |
dimer | 2 * 54045, recombinant enzyme, deduced from nucleotide sequence | Salmonella enterica |
Synonyms | Comment | Organism |
---|---|---|
GDP-alpha-D-mannose pyrophosphorylase | - |
Salmonella enterica |
RfbM | enzyme is encoded by the rfbM gene | Salmonella enterica |
RfbM | - |
Salmonella enterica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
GDP-mannose | competitive vs. GTP | Salmonella enterica | |
0.12 | - |
GDP-mannose | uncompetitive vs. mannose 1-phosphate | Salmonella enterica |