Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by dithiothreitol up to 5 mM | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0142 | - |
GDPmannose | pH 7.0, 25°C | Pseudomonas aeruginosa | |
0.0205 | - |
alpha-D-mannose 1-phosphate | pH 8.0, 25°C | Pseudomonas aeruginosa | |
0.0295 | - |
GTP | pH 8.0, 25°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ or Mn2+ required for activity | Pseudomonas aeruginosa | |
Mn2+ | Mg2+ or Mn2+ required for activity | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
gel filtration, phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase is a bifunctional enzyme catalyzing both the phosphomannose isomerase, i.e. PIM, and guanosine 5'-diphospho-D-mannose pyrophosphorylase, i.e. GMP, reaction | Pseudomonas aeruginosa |
56000 | - |
1 * 56000, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | Pseudomonas aeruginosa | enzyme of alginate biosynthetic pathway | GDPmannose + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P07874 | phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase is a bifunctional enzyme catalyzing both phosphomannose isomerase, i.e. PIM, and guanosine 5'-diphospho-D-mannose pyrophosphorylase, i.e. GMP, reactions | - |
Purification (Comment) | Organism |
---|---|
Biogel, Q-Sepharose, Sephacryl-200 | Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose | enzyme has both phosphomannose isomerase, i.e. PIM, and guanosine 5'-diphospho-D-mannose pyrophosphorylase, i.e. GMP, activity | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | no activity with GDP-D-glucose, ADP-D-mannose and UDP-D-mannose | Pseudomonas aeruginosa | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | enzyme of alginate biosynthetic pathway | Pseudomonas aeruginosa | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | - |
Pseudomonas aeruginosa | diphosphate + GDP-mannose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 56000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
PIM-GMP | - |
- |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |