Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | expression of cytHPPK/DHPS mRNA is induced by salt stress | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Saccharomyces cerevisiae | Arabidopsis thaliana |
isozyme cytHPPK/DHPS DNA and amino acid sequence determination and analysis, and phylogenetic analysis, transient expression of GFP-tagged isozyme cytHPPK/DHPS in the cytosol of Arabidopsis thaliana protoplasts using transfection via Agrobacterium tumefaciens strain GV3101/pMP90, functional complementation of a Saccharomyces cerevisiae mutant, that lacks the enzyme activity, by expression of isozyme cytHPPK/DHPS, expression and subcloning of N-terminally His6-tagged isozyme cytHPPK/DHPS in Escherichia coli strains DH-5alpha and BL-21AI, co-expression with GroESL chaperones | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a T-DNA insertion mutant in the cytHPPK/DHPS gene, resulting in lower germination rates as compared with the wild-type upon application of oxidative and osmotic stress, phenotype, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
cytosol | isozyme cytHPPK/DHPS lacking a potential transit peptide | Arabidopsis thaliana | 5829 | - |
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
mitochondrion | mitHPPK/DHPS | Arabidopsis thaliana | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54053 | - |
x * 54053, sequence calculation | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-hydroxymethyl-7,8-dihydropterin + ATP | Arabidopsis thaliana | tetrahydrofolate biosynthesis | 6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP | - |
? | |
ATP + 6-hydroxymethyl-7,8-dihydropterin | Arabidopsis thaliana | 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase, i.e. mitHPPK/DHPS, is a bifunctional mitochondrial enzyme, which catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis, folate biosynthesis in Arabidopsis thaliana, overview | AMP + (7,8-dihydropterin-6-yl)methyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | Q1ENB6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged isozyme cytHPPK/DHPS from Escherichia coli strains DH-5alpha and BL-21AI | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | ubiquitous expression of isozyme mitHPPK/DHPS | Arabidopsis thaliana | - |
seed | developing, isozyme cytHPPK/DHPS gene is exclusively expressed in developing seeds, histochemical analysis of a transgenic cytHPPK/DHPS promoter-GUS line | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-hydroxymethyl-7,8-dihydropterin + ATP | tetrahydrofolate biosynthesis | Arabidopsis thaliana | 6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP | - |
? | |
ATP + 6-hydroxymethyl-7,8-dihydropterin | - |
Arabidopsis thaliana | AMP + (7,8-dihydropterin-6-yl)methyl diphosphate | - |
? | |
ATP + 6-hydroxymethyl-7,8-dihydropterin | 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase, i.e. mitHPPK/DHPS, is a bifunctional mitochondrial enzyme, which catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis, folate biosynthesis in Arabidopsis thaliana, overview | Arabidopsis thaliana | AMP + (7,8-dihydropterin-6-yl)methyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54053, sequence calculation | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) | - |
Arabidopsis thaliana |
cytHPPK/DHPS | - |
Arabidopsis thaliana |
hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase | - |
Arabidopsis thaliana |
hydroxymethyldihydropterin pyrophosphokinase (HPPK) | - |
Arabidopsis thaliana |
mitHPPK/DHPS | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |