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Literature summary for 2.7.6.3 extracted from
- A rapid assay for dihydropteroate synthase activity suitable for identification of inhibitors (2007), Anal. Biochem., 360, 227-234.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharomyces cerevisiae |
| expression of His6-tagged enzyme in Escherichia coli | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dapsone | - |
Saccharomyces cerevisiae |  |
| sulfachloropyridazine | - |
Saccharomyces cerevisiae | |
| sulfamethoxazole | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, HPPK-DHPS coupled assay method, overview | Saccharomyces cerevisiae | |
| 0.00039 | - |
6-hydroxymethyl-7,8-dihydropterin | - |
Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-hydroxymethyl-7,8-dihydropterin + ATP | Saccharomyces cerevisiae | folate biosynthesis | 6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP | - |
? | |
| ATP + 6-hydroxymethyl-7,8-dihydropterin | Saccharomyces cerevisiae | step in the folate biosynthetic pathway, overview | AMP + (7,8-dihydropterin-6-yl)methyl diphosphate | - |
? | |
| additional information | Saccharomyces cerevisiae | the bifunctional HPPK-DHPS enzyme catalyzes sequential steps in the folate biosynthesis, i.e. the 6-hydroxymethylpterin pyrophosphokinase reaction and the dihydropteroate synthase, DHPS, reaction, EC 2.5.1.15 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P53848 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli by metal affinity chromatography and ion exchange chromatography, cleavage of the tag by thrombin | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-hydroxymethyl-7,8-dihydropterin + ATP | folate biosynthesis | Saccharomyces cerevisiae | 6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP | - |
? | |
| ATP + 6-hydroxymethyl-7,8-dihydropterin | - |
Saccharomyces cerevisiae | AMP + (7,8-dihydropterin-6-yl)methyl diphosphate | - |
? | |
| ATP + 6-hydroxymethyl-7,8-dihydropterin | step in the folate biosynthetic pathway, overview | Saccharomyces cerevisiae | AMP + (7,8-dihydropterin-6-yl)methyl diphosphate | - |
? | |
| additional information | the bifunctional HPPK-DHPS enzyme catalyzes sequential steps in the folate biosynthesis, i.e. the 6-hydroxymethylpterin pyrophosphokinase reaction and the dihydropteroate synthase, DHPS, reaction, EC 2.5.1.15 | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | coupled assay method development using reduction of DHPS reaction product dihydropteroate to tetrahydropteroate by excess dihydrofolate reductase with the cofactor NADPH, the oxidation of NADPH is monitored, overview | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 6-hydroxymethylpterin pyrophosphokinase | - |
Saccharomyces cerevisiae |
| HPPK | - |
Saccharomyces cerevisiae |
| HPPK-DHPS | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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