Literature summary for 2.7.6.3 extracted from

Mouillon, J.M.; Ravanel, S.; Douce, R.; Rebeille, F.
Folate synthesis in higher-plant mitochondria: coupling between the dihydropterin pyrophosphokinase and the dihydropteroate synthase activities (2002), Biochem. J., 363, 313-319.

Search for more literature for 2.7.6.3

Cloned(Commentary)

Cloned (Comment)	Organism
bifunctional protein 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase is involved in tetrahydrofolate expressed in Escherichia coli	Pisum sativum

Inhibitors

Inhibitors	Comment	Organism	Structure
6-hydroxymethyl-7,8-dihydropterin diphosphate	competitive inhibitor of ATP, mixed type inhibitor of 6-hydroxymethyl-7,8-dihydropteridine	Pisum sativum
AMP	poor inhibitor	Pisum sativum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.001	-	6-hydroxymethyl-7,8-dihydropteridine	bifunctional enzyme 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase	Pisum sativum
0.002	-	6-hydroxymethyl-7,8-dihydropteridine	recombinant bifunctional enzyme 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase expressed in Escherichia coli	Pisum sativum
0.07	-	ATP	bifunctional enzyme 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase from Pisum sativum and recombinant enzyme expressed in Escherichia coli	Pisum sativum

Organism

Organism	UniProt	Comment	Textmining
Pisum sativum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant bifunctional protein 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase is involved in tetrahydrofolate	Pisum sativum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 6-hydroxymethyl-7,8-dihydropteridine	ordered bi bi mechanism where ATP binds first and 6-hydroxymethyl-7,8-dihydropterin diphosphate is released last	Pisum sativum	AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate	-	?

Subunits

Subunits	Comment	Organism
More	the bifunctional protein 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase is involved in tetrahydrofolate synthesis	Pisum sativum

Synonyms

Synonyms	Comment	Organism
6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase	bifunctional protein involved in tetrahydrofolate synthesis	Pisum sativum
HPPK/DHPS	-	Pisum sativum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.005	-	6-hydroxymethyl-7,8-dihydropterin diphosphate	versus ATP	Pisum sativum
0.013	-	6-hydroxymethyl-7,8-dihydropterin diphosphate	versus 6-hydroxymethyl-7,8-dihydropteridine	Pisum sativum
0.4	-	AMP	versus 6-hydroxymethyl-7,8-dihydropteridine	Pisum sativum
0.7	-	AMP	versus ATP	Pisum sativum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)