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Literature summary for 2.7.6.2 extracted from
- Isolation and characterization of thiamin pyrophosphotransferase from Glycine max seedlings (1980), Plant Physiol., 66, 308-312.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | 0.5 mM, 8% inhibition | Glycine max |  |
| diphosphate | 0.5 mM, only 3% inhibition | Glycine max | |
| oxythiamine | 0.5 mM; 4% inhibition | Glycine max | |
| PCMB | 0.5 mM, 36% inhibition | Glycine max | |
| pyrithiamine | 0.5 mM, 77% inhibition | Glycine max | |
| thiamine diphosphate | 0.5 mM; 95% inhibition | Glycine max | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00464 | - |
thiamine | pH 8.1, 37°C | Glycine max | |
| 2.6 | - |
GTP | pH 8.1, 37°C | Glycine max | |
| 7.6 | - |
CTP | pH 8.1, 37°C | Glycine max | |
| 11.2 | - |
UTP | pH 8.1, 37°C | Glycine max | |
| 18.3 | - |
ATP | pH 8.1, 37°C | Glycine max | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
gel filtration | Glycine max |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Glycine max | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000061 | - |
- |
Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + thiamine | - |
Glycine max | AMP + thiamine diphosphate | - |
? | |
| CTP + thiamine | - |
Glycine max | CMP + thiamine diphosphate | - |
? | |
| GTP + thiamine | - |
Glycine max | GMP + thiamine diphosphate | - |
? | |
| UTP + thiamine | - |
Glycine max | UMP + thiamine diphosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | 7.3 | - |
Glycine max |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 8 | pH 6.4: about 40% of maximal activity, pH 8.0: about 50% of maximal activity | Glycine max |
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