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Literature summary for 2.7.4.8 extracted from

  • Jain, R.; Khan, N.; Menzel, A.; Rajkovic, I.; Konrad, M.; Techert, S.
    Insights into open/closed conformations of the catalytically active human guanylate kinase as investigated by small-angle X-ray scattering (2016), Eur. Biophys. J., 45, 81-89.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the N-terminally His6-tagged and SUMO-tagged enzyme in Escherichia coli strain BL21-(DE3)-pLysS Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
P1 -(5'-adenosyl)-P5 -(5'-guanosyl)pentaphosphate a non-hydrolysable bi-substrate analogue Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + GMP Homo sapiens
-
ADP + GDP
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16774 gene GUK1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged and SUMO-tagged enzyme from Escherichia coli strain BL21-(DE3)-pLysS by nickel affinity chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GMP
-
Homo sapiens ADP + GDP
-
?

Subunits

Subunits Comment Organism
? x * 22000, recombinant His6-SUMO-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
ATP:GMP phosphotransferase
-
Homo sapiens
GMPK
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens

General Information

General Information Comment Organism
evolution GMPK is a member of the family of ATP:NMP phosphoryltransferases, nucleoside monophosphate kinases, NMP kinases, or NMPKs Homo sapiens
additional information size and shape of open and closed enzyme GMPK are tracked by SAXS. The binding of substrates GMP and AMPPNP, or Ap5G, or GMP and ADP, results in the compaction of size and shape of human enzyme GMPK. Determination of structural changes between open and completely closed hGMPK conformation, overview. Homology modelling of hGMPK by using the crystal structure of mGMPK's closed conformation, residues 5-194 of 197, as template, PDB ID 1LVG Homo sapiens
physiological function enzyme GMPK plays an important role in the recycling of the secondary messenger cGMP and thereby regulates the supply of guanine nucleotides to various signal transduction pathways. In addition to its physiological roles, GMPK is also required for the intracellular activation of numerous antiviral and anticancer purine nucleoside analog prodrugs Homo sapiens