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Literature summary for 2.7.4.8 extracted from
- Purification and properties of guanylate kinase of mitochondrias from tubers of Jerusalem artichoke (1990), Plant Physiol. Biochem., 28, 191-201.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | free form, substrate inhibition, competitive toward MgATP2- | Helianthus tuberosus |  |
| GDP | GMP-phosphorylation | Helianthus tuberosus | |
| GTP | GMP-phosphorylation | Helianthus tuberosus | |
| K+ | weak | Helianthus tuberosus | |
| additional information | guanylic nucleotides strongly inhibit, compete with GMP | Helianthus tuberosus | |
| phosphate | weak | Helianthus tuberosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
GMP | - |
Helianthus tuberosus | |
| 0.42 | - |
MgATP2- | - |
Helianthus tuberosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Helianthus tuberosus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can partially replace Mg2+ | Helianthus tuberosus | |
| Fe2+ | activation, 90% as effective as Mg2+ | Helianthus tuberosus | |
| Mg2+ | requirement | Helianthus tuberosus | |
| Mg2+ | active substrate: MgATP2- | Helianthus tuberosus | |
| Mn2+ | requirement | Helianthus tuberosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
gel filtration | Helianthus tuberosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus tuberosus | - |
Jerusalem artichoke | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| method that includes DEAE-Sephacel and Blue Sepharose CL 6B chromatography | Helianthus tuberosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tuber | - |
Helianthus tuberosus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -25°C, stable | Helianthus tuberosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + GMP | - |
Helianthus tuberosus | ADP + GDP | - |
? | |
| dATP + dGMP | - |
Helianthus tuberosus | dADP + dGDP | - |
? | |
| GMP + MgATP2- | - |
Helianthus tuberosus | MgADP- + GDP | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Helianthus tuberosus |
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