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Literature summary for 2.7.4.8 extracted from
- Guanylate kinase from Saccharomyces cerevisiae. Isolation and characterization, crystallization and preliminary X-ray analysis, amino acid sequence and comparison with adenylate kinases (1989), Eur. J. Biochem., 184, 433-443.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| octahedral bipyramids, preliminary X-ray analysis | Saccharomyces cerevisiae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharomyces cerevisiae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
gel filtration | Saccharomyces cerevisiae |
| 20548 | - |
1 * 20548, calculated from amino acid sequence | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| method that includes DEAE-Sephacel, Cibacron-blue Sepharose and two Sephadex-75 chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 571 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, in N-ethylmorpholine-HCl buffer, pH 7.5, several years | Saccharomyces cerevisiae |
| 4°C, in N-ethylmorpholine-HCl buffer, pH 7.5, several years | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dGMP | - |
Saccharomyces cerevisiae | ADP + dGDP | - |
r | |
| ATP + GMP | best substrates | Saccharomyces cerevisiae | ADP + GDP | - |
? | |
| ATP + GMP | AMP is no acceptor substrate | Saccharomyces cerevisiae | ADP + GDP | - |
? | |
| dATP + dGMP | - |
Saccharomyces cerevisiae | dADP + dGDP | - |
? | |
| dATP + GMP | - |
Saccharomyces cerevisiae | dADP + GDP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 20548, calculated from amino acid sequence | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8.6 | about 80% of maximal activity at pH 6.8 and 8.6, about 95% of maximal activity at pH 7.3 and 8 | Saccharomyces cerevisiae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharomyces cerevisiae | pH 7.7, 25ºC | - |
5.8 |
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Release 2023.1 (January 2023)
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