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Literature summary for 2.7.4.7 extracted from

  • Lewin, L.M.; Brown, G.M.
    The biosynthesis of thiamine. III. Mechanism of enzymatic formation of the pyrophosphate ester of 2-methyl-4-amino-5-hydroxymethylpyrimidine (1961), J. Biol. Chem., 236, 2768-2771.
No PubMed abstract available
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Inhibitors

Inhibitors Comment Organism Structure
p-hydroxymercuribenzoate 1 mM, complete inhibition Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Saccharomyces cerevisiae
Mn2+ may substitute for Mg2+ Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-methyl-4-amino-5-phosphomethylpyrimidine Saccharomyces cerevisiae specific for ATP ADP + 2-methyl-4-amino-5-diphosphomethylpyrimidine product demonstrated in crude extract ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-methyl-4-amino-5-phosphomethylpyrimidine specific for ATP Saccharomyces cerevisiae ADP + 2-methyl-4-amino-5-diphosphomethylpyrimidine product demonstrated in crude extract ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Saccharomyces cerevisiae