Cloned (Comment) | Organism |
---|---|
- |
Strongylocentrotus purpuratus |
recombinant expression of full-length enzyme and each of its three catalytic domains | Strongylocentrotus purpuratus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant expression of full-length enzyme and each of its three catalytic domains. No enzymic activity of a sole catalytic domain | Strongylocentrotus purpuratus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 51% inhibition at 1 mM | Strongylocentrotus purpuratus | |
P1,P5-di(adenosine-5')pentaphosphate | 50% inhibition at 0.00041 mM, reactivation by 1 mM ADP if concentration of inhibitor is below 0.001 mM. No inhibition if 1 mM ADP is present; 50% inhibition at 0.00053 mM, recombinant enzyme; completely inhibits reactivation of flagella. 1 mM ADP prevents inhibition | Strongylocentrotus purpuratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
ADP | pH 7.6, 16°C, recombinant enzyme | Strongylocentrotus purpuratus | |
0.32 | - |
ADP | pH 7.6, 16°C, enzyme from sperm flagellum | Strongylocentrotus purpuratus | |
0.32 | - |
ADP | pH 7.6, isolated flagella | Strongylocentrotus purpuratus | |
0.32 | - |
ADP | pH 7.6, 16°C | Strongylocentrotus purpuratus | |
0.69 | - |
ADP | pH 7.6, 16°C, enzyme from embryonic cilia | Strongylocentrotus purpuratus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
flagellum | localizes along the entire flagellum, tightly bound to the axoneme. Enzyme contributes 31% of the total non-mitochondrial ATP synthesis associated with the demembranated axoneme | Strongylocentrotus purpuratus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
x * 130000, full-length enzyme, 21000, recombinant expression of catalytic domain 1 or 3, 22000, recombinant expression of catalytic domain 2, SDS-PAGE | Strongylocentrotus purpuratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Strongylocentrotus purpuratus | - |
- |
- |
Strongylocentrotus purpuratus | A2T1M5 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | n vitro phosphorylation results in 19% increase of vmax | Strongylocentrotus purpuratus |
Renatured (Comment) | Organism |
---|---|
reactivation of enzyme inhibited by P1,P5-di(adenosine-5)-pentaphosphate with 1 mM ADP if concentration of inhibitior is below 0.001 mM | Strongylocentrotus purpuratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | embryonic cilia | Strongylocentrotus purpuratus | - |
flagellate | most of protein is tightly bound to the axoneme | Strongylocentrotus purpuratus | - |
sperm flagellum | - |
Strongylocentrotus purpuratus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP | - |
Strongylocentrotus purpuratus | ATP + AMP | - |
? | |
ADP | - |
Strongylocentrotus purpuratus | ATP + AMP | - |
r | |
ADP | - |
Strongylocentrotus purpuratus | AMP + ATP | - |
? | |
ATP + AMP | - |
Strongylocentrotus purpuratus | 2 ADP | - |
r | |
additional information | at the measured in vivo concentrations of ADP of 0.114 mM, at pH 7.6, the axonemal adenylate kinase could contribute31%, and creatine kinase 69%, of the total non-mitochondrial ATP synthesis associated with the demembranated axoneme. The three catalytic domains of adenylate kinase are considerably divergent from each other | Strongylocentrotus purpuratus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 130000, full-length enzyme, 21000, recombinant expression of catalytic domain 1 or 3, 22000, recombinant expression of catalytic domain 2, SDS-PAGE | Strongylocentrotus purpuratus |
More | enzyme has three catalytic domains | Strongylocentrotus purpuratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
Strongylocentrotus purpuratus |
8.1 | - |
recombinant enzyme | Strongylocentrotus purpuratus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00041 | - |
pH 7.6, 16°C | Strongylocentrotus purpuratus | P1,P5-di(adenosine-5')pentaphosphate |