Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga neapolitana |
General Stability | Organism |
---|---|
more stable at high concentrations or in the presence of bovine serum albumin | Thermotoga neapolitana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: EDTA 10mM | Thermotoga neapolitana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0409 | - |
AMP | 30°C, pH 7.4 | Thermotoga neapolitana | |
0.0494 | - |
ATP | 30°C, pH 7.4 | Thermotoga neapolitana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 1 mol/mol recombinant enzyme | Thermotoga neapolitana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
1 * 25000, SDS-PAGE | Thermotoga neapolitana |
29000 | - |
gel filtration | Thermotoga neapolitana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga neapolitana | Q8GGL2 | - |
- |
Thermotoga neapolitana 5068 | Q8GGL2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga neapolitana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Vmax 1130 30°C | Thermotoga neapolitana |
1050 | - |
38°C | Thermotoga neapolitana |
8400 | - |
80°C | Thermotoga neapolitana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + AMP | - |
Thermotoga neapolitana | ADP + ADP | - |
? | |
ATP + AMP | - |
Thermotoga neapolitana 5068 | ADP + ADP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 25000, SDS-PAGE | Thermotoga neapolitana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermotoga neapolitana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
93.5 | - |
melting point EDTA-treated | Thermotoga neapolitana |
99.7 | - |
melting point holoenzyme | Thermotoga neapolitana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8 | - |
Thermotoga neapolitana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | - |
more than 70% active | Thermotoga neapolitana |
8.5 | - |
more than 70% active | Thermotoga neapolitana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
not at all stable at values below | Thermotoga neapolitana |
7.4 | 9.3 | 80% activity | Thermotoga neapolitana |