General Stability | Organism |
---|---|
dithiothreitol and 2-mercaptoethanol are not necessary as protecting agents | Vibrio natriegens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Vibrio natriegens | |
IAA | - |
Vibrio natriegens | |
N-ethylmaleimide | weak | Vibrio natriegens | |
p-chloromercuriphenylsulfonate | - |
Vibrio natriegens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
ATP | 30°C, pH 7.4 | Vibrio natriegens | |
0.114 | 0.13 | AMP | - |
Vibrio natriegens | |
0.45 | 0.55 | ADP | 30°C, pH 7.4 | Vibrio natriegens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Vibrio natriegens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Vibrio natriegens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
gel filtration | Vibrio natriegens |
32000 | - |
1 * 32000, SDS-PAGE | Vibrio natriegens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio natriegens | - |
Beneckea, type III | - |
Purification (Comment) | Organism |
---|---|
- |
Vibrio natriegens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1450 | - |
30°C, pH 7.4 | Vibrio natriegens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + ADP | - |
Vibrio natriegens | ATP + AMP | - |
r | |
ATP + AMP | no substrates are ATP/IMP | Vibrio natriegens | ADP + ADP | - |
r | |
ATP + AMP | no substrates are ATP/UMP | Vibrio natriegens | ADP + ADP | - |
r | |
ATP + AMP | no substrates are ATP/GMP | Vibrio natriegens | ADP + ADP | - |
r | |
ATP + dAMP | reaction at 46% the rate of AMP | Vibrio natriegens | ADP + dADP | - |
? | |
dATP + AMP | reaction at about 50% the rate of ATP | Vibrio natriegens | dADP + ADP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000, SDS-PAGE | Vibrio natriegens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Vibrio natriegens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
325 | - |
ATP | - |
Vibrio natriegens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.6 | broad | Vibrio natriegens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | less than 20% of maximal activity at pH 5, 5.5, 6 and pH 9.5 and 10, about half-maximal activity at pH 6.9 and 8.4 | Vibrio natriegens |
6.9 | 8.4 | about half-maximal activity at pH 6.9 and 8.4 | Vibrio natriegens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Vibrio natriegens | isoelectric focusing | 4.8 | 4.7 |