Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5'-guanylyl-imidodiphosphate | - |
Escherichia coli | |
GTP | - |
Escherichia coli |
Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D115A | little detrimental effect, activated by 5'-guanylyl-imidodiphosphate within a concentration range that is roughly similar to that of wild type enzyme | Escherichia coli |
H96A | involved in GTP activation, abolishing GTP activation | Escherichia coli |
N111A | little detrimental effect, activated by 5'-guanylyl-imidodiphosphate within a concentration range that is roughly similar to that of wild type enzyme | Escherichia coli |
R103A | involved in GTP activation, abolishing GTP activation | Escherichia coli |
R127A | decreasing affinity for GTP | Escherichia coli |
R130A | involved in GTP activation, abolishing GTP activation | Escherichia coli |
R92A | involved in GTP activation, abolishing GTP activation | Escherichia coli |
S124A | decreasing affinity for GTP | Escherichia coli |
W119A | involved in GTP activation, abolishing GTP activation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UTP | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A7E9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
R92A, H96A, and R127A exhibit increased enzyme activity | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
UMP kinase | - |
Escherichia coli |