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Literature summary for 2.7.4.22 extracted from

  • Tu, J.L.; Chin, K.H.; Wang, A.H.; Chou, S.H.
    The crystallization of apo-form UMP kinase from Xanthomonas campestris is significantly improved in a strong magnetic field (2007), Acta Crystallogr. Sect. F, F63, 438-442.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development since bacterial UMPKs are specific for the phosphorylation of UMP only and differ from eukaryotic dual-specificity UMP/CMP kinases Xanthomonas campestris pv. campestris

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3)-pLysS, overexpression of the Hig6-tagged target protein is induced by the addition of 0.5 mM IPTG at 20°C for 21 h, SeMet-substituted XC1936 is expressed in a similar way Xanthomonas campestris pv. campestris

Crystallization (Commentary)

Crystallization (Comment) Organism
apo-form UMPK, 2.35 A resolution, crystallisation is significantly improved in a strong magnetic field, buffer-screening procedure, 20 mM N-(2-acetamido)-2-iminodiacetic acid (ADA) pH 6.8, 5 mM beta-mercaptoethanol and 0.02% NaN3, sitting-drop vapour diffusion in 96-well plates, 100 K, monoclinic space group P212121, unit-cell paramerters a = 111.45, b = 120.07, c = 125.79A Xanthomonas campestris pv. campestris

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris pv. campestris
-
XC1936 gene fragment
-
Xanthomonas campestris pv. campestris 17
-
XC1936 gene fragment
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal-affinity chromatography (IMAC) on a nickel column, SDS-PAGE, further purified by FPLC on an anion-exchange column, greater than 99% purity Xanthomonas campestris pv. campestris

Subunits

Subunits Comment Organism
hexamer apo-form XC1936 Xanthomonas campestris pv. campestris

Synonyms

Synonyms Comment Organism
UMP kinase
-
Xanthomonas campestris pv. campestris
UMPK
-
Xanthomonas campestris pv. campestris
uridylate kinase
-
Xanthomonas campestris pv. campestris