Application | Comment | Organism |
---|---|---|
drug development | since bacterial UMPKs are specific for the phosphorylation of UMP only and differ from eukaryotic dual-specificity UMP/CMP kinases | Xanthomonas campestris pv. campestris |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3)-pLysS, overexpression of the Hig6-tagged target protein is induced by the addition of 0.5 mM IPTG at 20°C for 21 h, SeMet-substituted XC1936 is expressed in a similar way | Xanthomonas campestris pv. campestris |
Crystallization (Comment) | Organism |
---|---|
apo-form UMPK, 2.35 A resolution, crystallisation is significantly improved in a strong magnetic field, buffer-screening procedure, 20 mM N-(2-acetamido)-2-iminodiacetic acid (ADA) pH 6.8, 5 mM beta-mercaptoethanol and 0.02% NaN3, sitting-drop vapour diffusion in 96-well plates, 100 K, monoclinic space group P212121, unit-cell paramerters a = 111.45, b = 120.07, c = 125.79A | Xanthomonas campestris pv. campestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris pv. campestris | - |
XC1936 gene fragment | - |
Xanthomonas campestris pv. campestris 17 | - |
XC1936 gene fragment | - |
Purification (Comment) | Organism |
---|---|
immobilized metal-affinity chromatography (IMAC) on a nickel column, SDS-PAGE, further purified by FPLC on an anion-exchange column, greater than 99% purity | Xanthomonas campestris pv. campestris |
Subunits | Comment | Organism |
---|---|---|
hexamer | apo-form XC1936 | Xanthomonas campestris pv. campestris |
Synonyms | Comment | Organism |
---|---|---|
UMP kinase | - |
Xanthomonas campestris pv. campestris |
UMPK | - |
Xanthomonas campestris pv. campestris |
uridylate kinase | - |
Xanthomonas campestris pv. campestris |