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Literature summary for 2.7.4.22 extracted from

  • Evrin, C.; Straut, M.; Slavova-Azmanova, N.; Bucurenci, N.; Onu, A.; Assairi, L.; Ionescu, M.; Palibroda, N.; Barzu, O.; Gilles, A.
    Regulatory mechanisms differ in UMP kinases from Gram-negative and Gram-positive bacteria (2007), J. Biol. Chem., 282, 7242-7253.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
3'-anthraniloyl-dGTP
-
Haemophilus influenzae
3'-anthraniloyl-dGTP
-
Escherichia coli
3'-anthraniloyl-dGTP
-
Neisseria meningitidis
3'-anthraniloyl-dGTP strong activator Streptococcus pneumoniae
3'-anthraniloyl-dGTP strong activator Bacillus subtilis
7-deaza-dGTP
-
Haemophilus influenzae
7-deaza-dGTP
-
Streptococcus pneumoniae
7-deaza-dGTP
-
Bacillus subtilis
7-deaza-dGTP
-
Escherichia coli
7-deaza-dGTP
-
Neisseria meningitidis
dGTP
-
Haemophilus influenzae
dGTP
-
Streptococcus pneumoniae
dGTP
-
Bacillus subtilis
dGTP
-
Escherichia coli
dGTP
-
Neisseria meningitidis
GMP
-
Haemophilus influenzae
GMP not activating Streptococcus pneumoniae
GMP not activating Bacillus subtilis
GMP-PNP
-
Streptococcus pneumoniae
GMP-PNP
-
Bacillus subtilis
GMP-PNP
-
Escherichia coli
GMP-PNP GMP-PNP Haemophilus influenzae
GMP-PNP less activating Neisseria meningitidis
GTP 0.5 mM Staphylococcus aureus
GTP 0.5 mM Enterococcus faecalis
GTP 0.5 mM Streptococcus pneumoniae
GTP at 0.5 mM Salmonella enterica subsp. enterica serovar Typhimurium
GTP 0.5 mM, independent of the concentrations of Mg2+ Haemophilus influenzae
GTP 0.5 mM, more activating at low concentrations of ATP Bacillus subtilis
GTP 0.5 mM, reverses the inhibition of excess UMP, it increases the affinity for ATP Escherichia coli
GTP 0.5 mM, reverses the inhibition of excess UMP, it increases the affinity for ATP Neisseria meningitidis
ITP
-
Haemophilus influenzae
ITP
-
Streptococcus pneumoniae
ITP
-
Bacillus subtilis
ITP
-
Escherichia coli
ITP
-
Neisseria meningitidis
additional information GMP is not activating Neisseria meningitidis
XTP
-
Haemophilus influenzae
XTP
-
Streptococcus pneumoniae
XTP
-
Bacillus subtilis
XTP
-
Escherichia coli
XTP
-
Neisseria meningitidis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium
expression in Escherichia coli Staphylococcus aureus
expression in Escherichia coli Haemophilus influenzae
expression in Escherichia coli Enterococcus faecalis
expression in Escherichia coli Streptococcus pneumoniae
expression in Escherichia coli Neisseria meningitidis
expression in Escherichia coli, site directed mutagenesis Bacillus subtilis
expression in Escherichia coli, site directed mutagenesis Escherichia coli

Protein Variants

Protein Variants Comment Organism
N137A Tm 10°C lower than wild type, loss of cooperativity with ATP, sensitive to activation by GTP Bacillus subtilis
N140A cooperative inhibition by GTP and UTP is altered, lower thermodynamic stability Escherichia coli
T135A Tm 10°C lower than wild type, loss of cooperativity with ATP, increase in Km for UMP, sensitive to activation by GTP Bacillus subtilis
T135A/N137A Tm 10°C lower than wild type, at pH 7.4 in 50 mM Tris irreversible inactivated within hours Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
5-bromo-UMP not inhibitory Enterococcus faecalis
5-bromo-UMP not inhibitory Escherichia coli
5-bromo-UMP not inhibitory Haemophilus influenzae
5-bromo-UMP not inhibitory Streptococcus pneumoniae
5-Bromo-UTP
-
Bacillus subtilis
5-Bromo-UTP less inhibitory than UTP Enterococcus faecalis
5-Bromo-UTP less inhibitory than UTP Escherichia coli
5-Bromo-UTP less inhibitory than UTP Haemophilus influenzae
5-Bromo-UTP more inhibitory than UTP Streptococcus pneumoniae
5-fluoro-UTP
-
Bacillus subtilis
5-fluoro-UTP like UTP Enterococcus faecalis
5-fluoro-UTP like UTP Escherichia coli
5-fluoro-UTP like UTP Haemophilus influenzae
5-fluoro-UTP less inhibitory than UTP Streptococcus pneumoniae
5-iodo-UMP not inhibitory Enterococcus faecalis
5-iodo-UMP not inhibitory Escherichia coli
5-iodo-UMP not inhibitory Haemophilus influenzae
5-iodo-UMP not inhibitory Streptococcus pneumoniae
5-iodo-UTP
-
Bacillus subtilis
5-iodo-UTP less inhibitory than UTP Enterococcus faecalis
5-iodo-UTP less inhibitory than UTP Escherichia coli
5-iodo-UTP less inhibitory than UTP Haemophilus influenzae
5-iodo-UTP more inhibitory than UTP Streptococcus pneumoniae
dUTP five times weaker than UTP Bacillus subtilis
dUTP five times weaker than UTP Escherichia coli
dUTP five times weaker than UTP Haemophilus influenzae
dUTP five times weaker than UTP Streptococcus pneumoniae
TTP not inhibitory Bacillus subtilis
TTP not inhibitory Enterococcus faecalis
TTP not inhibitory Escherichia coli
TTP not inhibitory Haemophilus influenzae
TTP not inhibitory Streptococcus pneumoniae
UMP
-
Bacillus subtilis
UMP excess UMP at high concentrations, UTP deminishes this inhibition Escherichia coli
UMP excess UMP at high concentrations, UTP deminishes this inhibition Haemophilus influenzae
UMP excess UMP at high concentrations, UTP deminishes this inhibition Neisseria meningitidis
UMP excess UMP at high concentrations Streptococcus pneumoniae
UTP inhibition not affected by Mg2+ and UMP Bacillus subtilis
UTP inhibition not affected by Mg2+ and UMP Enterococcus faecalis
UTP 0.25 mM, 0.5 mM, GTP, UMP and high concentrations of Mg2+ protects against inhibition by UTP, decreases the affinity for ATP, inhibition only without Mg2+ Escherichia coli
UTP 0.25 mM, 0.5 mM, GTP, UMP and high concentrations of Mg2+ protects against inhibition by UTP, decreases the affinity for ATP, inhibition only without Mg2+ Haemophilus influenzae
UTP 0.25 mM, 0.5 mM, UMP protects against inhibition by UTP, decreases the affinity for ATP, inhibition only without Mg2+ Neisseria meningitidis
UTP
-
Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
UMP in presence of 2.0 mM ATP without GTP Neisseria meningitidis
0.01
-
UMP in presence of 2.0 mM ATP without GTP Bacillus subtilis
0.0156
-
UMP in presence of 10.0 mM ATP without GTP Neisseria meningitidis
0.0274
-
UMP in presence of 2.0 mM ATP and 0.5 mM GTP Bacillus subtilis
0.04
-
UMP in presence of 1.0 mM ATP and 0.5 mM GTP Haemophilus influenzae
0.04
-
UMP in presence of 1.0 mM ATP without GTP Haemophilus influenzae
0.046
-
UMP in presence of 2.0 mM ATP without GTP Escherichia coli
0.047
-
UMP in presence of 0.2 mM ATP and 0.5 mM GTP Escherichia coli
0.0492
-
UMP in presence of 0.2 mM ATP without GTP Escherichia coli
0.05
-
UMP in presence of 12.0 mM ATP and 0.5 mM GTP Haemophilus influenzae
0.051
-
UMP in presence of 2.0 mM ATP and 0.5 mM GTP Escherichia coli
0.0574
-
UMP in presence of 10.0 mM ATP and 0.5 mM GTP Neisseria meningitidis
0.0997
-
UMP in presence of 2.0 mM ATP and 0.5 mM GTP Streptococcus pneumoniae
0.1
-
UMP in presence of 12.0 mM ATP without GTP Haemophilus influenzae
0.1
-
UMP in presence of 2.0 mM ATP and 0.5 mM GTP Neisseria meningitidis
0.1056
-
UMP in presence of 30 mM ATP and 0.5 mM GTP Streptococcus pneumoniae
0.131
-
UMP in presence of 30 mM ATP without GTP Bacillus subtilis
0.15
-
ATP in presence of 1.0 mM UMP without GTP Escherichia coli
0.15
-
UMP in presence of 30 mM ATP without GTP Streptococcus pneumoniae
0.155
-
UMP in presence of 30 mM ATP and 0.5 mM GTP Bacillus subtilis
0.2
-
ATP in presence of 1.0 mM UMP and 0.5 mM GTP Escherichia coli
0.207
-
UMP in presence of 2.0 mM ATP, 0.5 mM UTP and 0.5 mM GTP Escherichia coli
0.23
-
ATP in presence of 0.1 mM UMP without GTP Escherichia coli
0.26
-
ATP in presence of 2.0 mM ATP, 0.5 mM UTP and 0.5 mM GTP Escherichia coli
0.27
-
ATP in presence of 0.1 mM UMP and 0.5 mM GTP Escherichia coli
0.46
-
ATP in presence of 0.1 mM UMP and 0.5 mM GTP Haemophilus influenzae
0.6
-
ATP in presence of 1.0 mM UMP and 0.5 mM GTP Haemophilus influenzae
0.77
-
ATP in presence of 2.0 mM ATP and 0.5 mM UTP Escherichia coli
0.83
-
ATP in presence of 0.05 mM UMP and 0.5 mM GTP Neisseria meningitidis
0.9
-
ATP in presence of 1.0 mM UMP and 0.5 mM GTP Staphylococcus aureus
0.92
-
ATP in presence of 1.0 mM UMP and 0.5 mM GTP Streptococcus pneumoniae
0.93
-
ATP in presence of 0.1 mM UMP and 0.5 mM GTP Bacillus subtilis
1
-
ATP in presence of 0.5 mM UTP Escherichia coli
1.57
-
ATP in presence of 0.1 mM UMP without GTP Haemophilus influenzae
1.62
-
ATP in presence of 1.0 mM UMP without GTP Haemophilus influenzae
1.69
-
UMP in presence of 2.0 mM ATP and 0.5 mM UTP Escherichia coli
1.79
-
ATP in presence of 1.0 mM UMP and 0.5 mM GTP Bacillus subtilis
1.98
-
ATP in presence of 1.0 mM UMP and 0.5 mM GTP Neisseria meningitidis
2.64
-
ATP in presence of 1.0 mM UMP without GTP Staphylococcus aureus
2.98
-
ATP in presence of 0.05 mM UMP without GTP Neisseria meningitidis
3.22
-
ATP in presence of 1.0 mM UMP without GTP Neisseria meningitidis
4.5
-
ATP in presence of 0.5 mM UTP Haemophilus influenzae
9.8
-
ATP in presence of 1.0 mM UMP without GTP Streptococcus pneumoniae
10.4
-
ATP in presence of 0.1 mM UMP without GTP Bacillus subtilis
14.5
-
ATP in presence of 1.0 mM UMP without GTP Bacillus subtilis
22.2
-
UMP in presence of 2.0 mM ATP without GTP Streptococcus pneumoniae
30
-
ATP in presence of 0.5 mM UTP Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+
-
Staphylococcus aureus
Mg2+
-
Haemophilus influenzae
Mg2+
-
Enterococcus faecalis
Mg2+
-
Streptococcus pneumoniae
Mg2+
-
Bacillus subtilis
Mg2+
-
Escherichia coli
Mg2+
-
Neisseria meningitidis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O31749
-
-
Enterococcus faecalis
-
-
-
Escherichia coli A7ZWB7
-
-
Haemophilus influenzae
-
-
-
Neisseria meningitidis P65932
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Staphylococcus aureus
-
-
-
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant protein by affinity chromatography Salmonella enterica subsp. enterica serovar Typhimurium
of the recombinant protein by affinity chromatography Staphylococcus aureus
of the recombinant protein by affinity chromatography Enterococcus faecalis
of the recombinant protein by affinity chromatography Streptococcus pneumoniae
of the recombinant protein by affinity chromatography Bacillus subtilis
of the recombinant protein by affinity chromatography Neisseria meningitidis
of the recombinant protein by affinity chromatography, no difference in activity compared to the wild type Haemophilus influenzae
of the recombinant protein by affinity chromatography, no difference in activity compared to the wild type Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
at 2.0 mM ATP, without GTP Enterococcus faecalis
2.7
-
at 2.0 mM ATP, without GTP Bacillus subtilis
3.7
-
at 2.0 mM ATP, without GTP Streptococcus pneumoniae
4.6
-
at 2.0 mM ATP, without GTP Staphylococcus aureus
5.1
-
activity dependent of concentrations of ATP, at 2.0 mM ATP, without GTP Neisseria meningitidis
9.1
-
activity independent of concentrations of ATP, at 2.0 mM ATP, without GTP Haemophilus influenzae
9.1
-
at 8.0 mM ATP, without GTP Enterococcus faecalis
9.7
-
at 2.0 mM ATP, with 0.5 mM GTP Enterococcus faecalis
10.2
-
at 8.0 mM ATP, with 0.5 mM GTP Neisseria meningitidis
11.3
-
at 8.0 mM ATP, without GTP Neisseria meningitidis
11.8
-
at 8.0 mM ATP, without GTP Haemophilus influenzae
12.8
-
at 8.0 mM ATP, without GTP Staphylococcus aureus
18.1
-
at 8.0 mM ATP, without GTP Bacillus subtilis
19.4
-
at 8.0 mM ATP, with 0.5 mM GTP Enterococcus faecalis
23.5
-
at 2.0 mM ATP, with 0.5 mM GTP Staphylococcus aureus
23.6
-
at 8.0 mM ATP, with 0.5 mM GTP Staphylococcus aureus
26.1
-
at 2.0 mM ATP, without GTP Escherichia coli
28.1
-
at 8.0 mM ATP, without GTP Escherichia coli
28.6
-
at 2.0 mM ATP, with 0.5 mM GTP Bacillus subtilis
38.9
-
at 8.0 mM ATP, without GTP Streptococcus pneumoniae
39.1
-
at 8.0 mM ATP, with 0.5 mM GTP Bacillus subtilis
46.8
-
activity independent of concentrations of ATP, at 2.0 mM ATP, without GTP Salmonella enterica subsp. enterica serovar Typhimurium
47.5
-
at 8.0 mM ATP, without GTP Salmonella enterica subsp. enterica serovar Typhimurium
52.8
-
at 2.0 mM ATP, with 0.5 mM GTP Haemophilus influenzae
57.7
-
at 2.0 mM ATP, with 0.5 mM GTP Neisseria meningitidis
60.5
-
at 8.0 mM ATP, with 0.5 mM GTP Haemophilus influenzae
65.9
-
at 2.0 mM ATP, with 0.5 mM GTP Escherichia coli
68.5
-
at 2.0 mM ATP, with 0.5 mM GTP Streptococcus pneumoniae
71.1
-
at 8.0 mM ATP, with 0.5 mM GTP Escherichia coli
87.4
-
at 8.0 mM ATP, with 0.5 mM GTP Streptococcus pneumoniae
124.2
-
at 8.0 mM ATP, with 0.5 mM GTP Salmonella enterica subsp. enterica serovar Typhimurium
134.3
-
at 2.0 mM ATP, with 0.5 mM GTP Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UMP
-
Salmonella enterica subsp. enterica serovar Typhimurium ADP + UDP
-
r
ATP + UMP
-
Staphylococcus aureus ADP + UDP
-
r
ATP + UMP
-
Haemophilus influenzae ADP + UDP
-
r
ATP + UMP
-
Enterococcus faecalis ADP + UDP
-
r
ATP + UMP
-
Streptococcus pneumoniae ADP + UDP
-
r
ATP + UMP
-
Bacillus subtilis ADP + UDP
-
r
ATP + UMP
-
Escherichia coli ADP + UDP
-
r
ATP + UMP
-
Neisseria meningitidis ADP + UDP
-
r

Subunits

Subunits Comment Organism
hexamer gel filtration Staphylococcus aureus
hexamer gel filtration Enterococcus faecalis
hexamer gel filtration Streptococcus pneumoniae
hexamer gel filtration Bacillus subtilis
hexamer gel filtration Neisseria meningitidis
hexamer gel permeation Salmonella enterica subsp. enterica serovar Typhimurium
hexamer gel permeation Haemophilus influenzae
hexamer gel permeation Escherichia coli

Synonyms

Synonyms Comment Organism
ATP:UMP phosphotransferase
-
Salmonella enterica subsp. enterica serovar Typhimurium
ATP:UMP phosphotransferase
-
Staphylococcus aureus
ATP:UMP phosphotransferase
-
Haemophilus influenzae
ATP:UMP phosphotransferase
-
Enterococcus faecalis
ATP:UMP phosphotransferase
-
Streptococcus pneumoniae
ATP:UMP phosphotransferase
-
Bacillus subtilis
ATP:UMP phosphotransferase
-
Escherichia coli
ATP:UMP phosphotransferase
-
Neisseria meningitidis
UMP kinase
-
Salmonella enterica subsp. enterica serovar Typhimurium
UMP kinase
-
Staphylococcus aureus
UMP kinase
-
Haemophilus influenzae
UMP kinase
-
Enterococcus faecalis
UMP kinase
-
Streptococcus pneumoniae
UMP kinase
-
Bacillus subtilis
UMP kinase
-
Escherichia coli
UMP kinase
-
Neisseria meningitidis
uridine monophosphate kinase
-
Salmonella enterica subsp. enterica serovar Typhimurium
uridine monophosphate kinase
-
Staphylococcus aureus
uridine monophosphate kinase
-
Haemophilus influenzae
uridine monophosphate kinase
-
Enterococcus faecalis
uridine monophosphate kinase
-
Streptococcus pneumoniae
uridine monophosphate kinase
-
Bacillus subtilis
uridine monophosphate kinase
-
Neisseria meningitidis
uridylate kinase
-
Salmonella enterica subsp. enterica serovar Typhimurium
uridylate kinase
-
Staphylococcus aureus
uridylate kinase
-
Haemophilus influenzae
uridylate kinase
-
Enterococcus faecalis
uridylate kinase
-
Streptococcus pneumoniae
uridylate kinase
-
Bacillus subtilis
uridylate kinase
-
Neisseria meningitidis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.11
-
UMP at 2.0 mM ATP, without GTP Neisseria meningitidis
0.17
-
UMP at 12 mM ATP, without GTP Haemophilus influenzae
0.23
-
UMP at 10 mM ATP, without GTP Neisseria meningitidis
0.31
-
UMP at 1.0 mM ATP, without GTP Haemophilus influenzae
0.44
-
UMP at 2.0 mM ATP, without GTP Escherichia coli
0.6
-
UMP at 2.0 mM ATP, without GTP Escherichia coli
0.66
-
UMP at 2.0 mM ATP, without GTP, no inhibition at 30 mM ATP Bacillus subtilis
4.4
-
UMP at 2.0 mM ATP, without GTP Streptococcus pneumoniae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.01
-
at 0.05 mM UMP, UMP protects for inhibition by Mg2+ free UTP Neisseria meningitidis UTP
0.13
-
at 0.2 mM UMP, UMP protects for inhibition by Mg2+ free UTP Neisseria meningitidis UTP