Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3'-anthraniloyl-dGTP | - |
Haemophilus influenzae | |
3'-anthraniloyl-dGTP | - |
Escherichia coli | |
3'-anthraniloyl-dGTP | - |
Neisseria meningitidis | |
3'-anthraniloyl-dGTP | strong activator | Streptococcus pneumoniae | |
3'-anthraniloyl-dGTP | strong activator | Bacillus subtilis | |
7-deaza-dGTP | - |
Haemophilus influenzae | |
7-deaza-dGTP | - |
Streptococcus pneumoniae | |
7-deaza-dGTP | - |
Bacillus subtilis | |
7-deaza-dGTP | - |
Escherichia coli | |
7-deaza-dGTP | - |
Neisseria meningitidis | |
dGTP | - |
Haemophilus influenzae | |
dGTP | - |
Streptococcus pneumoniae | |
dGTP | - |
Bacillus subtilis | |
dGTP | - |
Escherichia coli | |
dGTP | - |
Neisseria meningitidis | |
GMP | - |
Haemophilus influenzae | |
GMP | not activating | Streptococcus pneumoniae | |
GMP | not activating | Bacillus subtilis | |
GMP-PNP | - |
Streptococcus pneumoniae | |
GMP-PNP | - |
Bacillus subtilis | |
GMP-PNP | - |
Escherichia coli | |
GMP-PNP | GMP-PNP | Haemophilus influenzae | |
GMP-PNP | less activating | Neisseria meningitidis | |
GTP | 0.5 mM | Staphylococcus aureus | |
GTP | 0.5 mM | Enterococcus faecalis | |
GTP | 0.5 mM | Streptococcus pneumoniae | |
GTP | at 0.5 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
GTP | 0.5 mM, independent of the concentrations of Mg2+ | Haemophilus influenzae | |
GTP | 0.5 mM, more activating at low concentrations of ATP | Bacillus subtilis | |
GTP | 0.5 mM, reverses the inhibition of excess UMP, it increases the affinity for ATP | Escherichia coli | |
GTP | 0.5 mM, reverses the inhibition of excess UMP, it increases the affinity for ATP | Neisseria meningitidis | |
ITP | - |
Haemophilus influenzae | |
ITP | - |
Streptococcus pneumoniae | |
ITP | - |
Bacillus subtilis | |
ITP | - |
Escherichia coli | |
ITP | - |
Neisseria meningitidis | |
additional information | GMP is not activating | Neisseria meningitidis | |
XTP | - |
Haemophilus influenzae | |
XTP | - |
Streptococcus pneumoniae | |
XTP | - |
Bacillus subtilis | |
XTP | - |
Escherichia coli | |
XTP | - |
Neisseria meningitidis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
expression in Escherichia coli | Staphylococcus aureus |
expression in Escherichia coli | Haemophilus influenzae |
expression in Escherichia coli | Enterococcus faecalis |
expression in Escherichia coli | Streptococcus pneumoniae |
expression in Escherichia coli | Neisseria meningitidis |
expression in Escherichia coli, site directed mutagenesis | Bacillus subtilis |
expression in Escherichia coli, site directed mutagenesis | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
N137A | Tm 10°C lower than wild type, loss of cooperativity with ATP, sensitive to activation by GTP | Bacillus subtilis |
N140A | cooperative inhibition by GTP and UTP is altered, lower thermodynamic stability | Escherichia coli |
T135A | Tm 10°C lower than wild type, loss of cooperativity with ATP, increase in Km for UMP, sensitive to activation by GTP | Bacillus subtilis |
T135A/N137A | Tm 10°C lower than wild type, at pH 7.4 in 50 mM Tris irreversible inactivated within hours | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-bromo-UMP | not inhibitory | Enterococcus faecalis | |
5-bromo-UMP | not inhibitory | Escherichia coli | |
5-bromo-UMP | not inhibitory | Haemophilus influenzae | |
5-bromo-UMP | not inhibitory | Streptococcus pneumoniae | |
5-Bromo-UTP | - |
Bacillus subtilis | |
5-Bromo-UTP | less inhibitory than UTP | Enterococcus faecalis | |
5-Bromo-UTP | less inhibitory than UTP | Escherichia coli | |
5-Bromo-UTP | less inhibitory than UTP | Haemophilus influenzae | |
5-Bromo-UTP | more inhibitory than UTP | Streptococcus pneumoniae | |
5-fluoro-UTP | - |
Bacillus subtilis | |
5-fluoro-UTP | like UTP | Enterococcus faecalis | |
5-fluoro-UTP | like UTP | Escherichia coli | |
5-fluoro-UTP | like UTP | Haemophilus influenzae | |
5-fluoro-UTP | less inhibitory than UTP | Streptococcus pneumoniae | |
5-iodo-UMP | not inhibitory | Enterococcus faecalis | |
5-iodo-UMP | not inhibitory | Escherichia coli | |
5-iodo-UMP | not inhibitory | Haemophilus influenzae | |
5-iodo-UMP | not inhibitory | Streptococcus pneumoniae | |
5-iodo-UTP | - |
Bacillus subtilis | |
5-iodo-UTP | less inhibitory than UTP | Enterococcus faecalis | |
5-iodo-UTP | less inhibitory than UTP | Escherichia coli | |
5-iodo-UTP | less inhibitory than UTP | Haemophilus influenzae | |
5-iodo-UTP | more inhibitory than UTP | Streptococcus pneumoniae | |
dUTP | five times weaker than UTP | Bacillus subtilis | |
dUTP | five times weaker than UTP | Escherichia coli | |
dUTP | five times weaker than UTP | Haemophilus influenzae | |
dUTP | five times weaker than UTP | Streptococcus pneumoniae | |
TTP | not inhibitory | Bacillus subtilis | |
TTP | not inhibitory | Enterococcus faecalis | |
TTP | not inhibitory | Escherichia coli | |
TTP | not inhibitory | Haemophilus influenzae | |
TTP | not inhibitory | Streptococcus pneumoniae | |
UMP | - |
Bacillus subtilis | |
UMP | excess UMP at high concentrations, UTP deminishes this inhibition | Escherichia coli | |
UMP | excess UMP at high concentrations, UTP deminishes this inhibition | Haemophilus influenzae | |
UMP | excess UMP at high concentrations, UTP deminishes this inhibition | Neisseria meningitidis | |
UMP | excess UMP at high concentrations | Streptococcus pneumoniae | |
UTP | inhibition not affected by Mg2+ and UMP | Bacillus subtilis | |
UTP | inhibition not affected by Mg2+ and UMP | Enterococcus faecalis | |
UTP | 0.25 mM, 0.5 mM, GTP, UMP and high concentrations of Mg2+ protects against inhibition by UTP, decreases the affinity for ATP, inhibition only without Mg2+ | Escherichia coli | |
UTP | 0.25 mM, 0.5 mM, GTP, UMP and high concentrations of Mg2+ protects against inhibition by UTP, decreases the affinity for ATP, inhibition only without Mg2+ | Haemophilus influenzae | |
UTP | 0.25 mM, 0.5 mM, UMP protects against inhibition by UTP, decreases the affinity for ATP, inhibition only without Mg2+ | Neisseria meningitidis | |
UTP | - |
Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0087 | - |
UMP | in presence of 2.0 mM ATP without GTP | Neisseria meningitidis | |
0.01 | - |
UMP | in presence of 2.0 mM ATP without GTP | Bacillus subtilis | |
0.0156 | - |
UMP | in presence of 10.0 mM ATP without GTP | Neisseria meningitidis | |
0.0274 | - |
UMP | in presence of 2.0 mM ATP and 0.5 mM GTP | Bacillus subtilis | |
0.04 | - |
UMP | in presence of 1.0 mM ATP and 0.5 mM GTP | Haemophilus influenzae | |
0.04 | - |
UMP | in presence of 1.0 mM ATP without GTP | Haemophilus influenzae | |
0.046 | - |
UMP | in presence of 2.0 mM ATP without GTP | Escherichia coli | |
0.047 | - |
UMP | in presence of 0.2 mM ATP and 0.5 mM GTP | Escherichia coli | |
0.0492 | - |
UMP | in presence of 0.2 mM ATP without GTP | Escherichia coli | |
0.05 | - |
UMP | in presence of 12.0 mM ATP and 0.5 mM GTP | Haemophilus influenzae | |
0.051 | - |
UMP | in presence of 2.0 mM ATP and 0.5 mM GTP | Escherichia coli | |
0.0574 | - |
UMP | in presence of 10.0 mM ATP and 0.5 mM GTP | Neisseria meningitidis | |
0.0997 | - |
UMP | in presence of 2.0 mM ATP and 0.5 mM GTP | Streptococcus pneumoniae | |
0.1 | - |
UMP | in presence of 12.0 mM ATP without GTP | Haemophilus influenzae | |
0.1 | - |
UMP | in presence of 2.0 mM ATP and 0.5 mM GTP | Neisseria meningitidis | |
0.1056 | - |
UMP | in presence of 30 mM ATP and 0.5 mM GTP | Streptococcus pneumoniae | |
0.131 | - |
UMP | in presence of 30 mM ATP without GTP | Bacillus subtilis | |
0.15 | - |
ATP | in presence of 1.0 mM UMP without GTP | Escherichia coli | |
0.15 | - |
UMP | in presence of 30 mM ATP without GTP | Streptococcus pneumoniae | |
0.155 | - |
UMP | in presence of 30 mM ATP and 0.5 mM GTP | Bacillus subtilis | |
0.2 | - |
ATP | in presence of 1.0 mM UMP and 0.5 mM GTP | Escherichia coli | |
0.207 | - |
UMP | in presence of 2.0 mM ATP, 0.5 mM UTP and 0.5 mM GTP | Escherichia coli | |
0.23 | - |
ATP | in presence of 0.1 mM UMP without GTP | Escherichia coli | |
0.26 | - |
ATP | in presence of 2.0 mM ATP, 0.5 mM UTP and 0.5 mM GTP | Escherichia coli | |
0.27 | - |
ATP | in presence of 0.1 mM UMP and 0.5 mM GTP | Escherichia coli | |
0.46 | - |
ATP | in presence of 0.1 mM UMP and 0.5 mM GTP | Haemophilus influenzae | |
0.6 | - |
ATP | in presence of 1.0 mM UMP and 0.5 mM GTP | Haemophilus influenzae | |
0.77 | - |
ATP | in presence of 2.0 mM ATP and 0.5 mM UTP | Escherichia coli | |
0.83 | - |
ATP | in presence of 0.05 mM UMP and 0.5 mM GTP | Neisseria meningitidis | |
0.9 | - |
ATP | in presence of 1.0 mM UMP and 0.5 mM GTP | Staphylococcus aureus | |
0.92 | - |
ATP | in presence of 1.0 mM UMP and 0.5 mM GTP | Streptococcus pneumoniae | |
0.93 | - |
ATP | in presence of 0.1 mM UMP and 0.5 mM GTP | Bacillus subtilis | |
1 | - |
ATP | in presence of 0.5 mM UTP | Escherichia coli | |
1.57 | - |
ATP | in presence of 0.1 mM UMP without GTP | Haemophilus influenzae | |
1.62 | - |
ATP | in presence of 1.0 mM UMP without GTP | Haemophilus influenzae | |
1.69 | - |
UMP | in presence of 2.0 mM ATP and 0.5 mM UTP | Escherichia coli | |
1.79 | - |
ATP | in presence of 1.0 mM UMP and 0.5 mM GTP | Bacillus subtilis | |
1.98 | - |
ATP | in presence of 1.0 mM UMP and 0.5 mM GTP | Neisseria meningitidis | |
2.64 | - |
ATP | in presence of 1.0 mM UMP without GTP | Staphylococcus aureus | |
2.98 | - |
ATP | in presence of 0.05 mM UMP without GTP | Neisseria meningitidis | |
3.22 | - |
ATP | in presence of 1.0 mM UMP without GTP | Neisseria meningitidis | |
4.5 | - |
ATP | in presence of 0.5 mM UTP | Haemophilus influenzae | |
9.8 | - |
ATP | in presence of 1.0 mM UMP without GTP | Streptococcus pneumoniae | |
10.4 | - |
ATP | in presence of 0.1 mM UMP without GTP | Bacillus subtilis | |
14.5 | - |
ATP | in presence of 1.0 mM UMP without GTP | Bacillus subtilis | |
22.2 | - |
UMP | in presence of 2.0 mM ATP without GTP | Streptococcus pneumoniae | |
30 | - |
ATP | in presence of 0.5 mM UTP | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Mg2+ | - |
Staphylococcus aureus | |
Mg2+ | - |
Haemophilus influenzae | |
Mg2+ | - |
Enterococcus faecalis | |
Mg2+ | - |
Streptococcus pneumoniae | |
Mg2+ | - |
Bacillus subtilis | |
Mg2+ | - |
Escherichia coli | |
Mg2+ | - |
Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O31749 | - |
- |
Enterococcus faecalis | - |
- |
- |
Escherichia coli | A7ZWB7 | - |
- |
Haemophilus influenzae | - |
- |
- |
Neisseria meningitidis | P65932 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Staphylococcus aureus | - |
- |
- |
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of the recombinant protein by affinity chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
of the recombinant protein by affinity chromatography | Staphylococcus aureus |
of the recombinant protein by affinity chromatography | Enterococcus faecalis |
of the recombinant protein by affinity chromatography | Streptococcus pneumoniae |
of the recombinant protein by affinity chromatography | Bacillus subtilis |
of the recombinant protein by affinity chromatography | Neisseria meningitidis |
of the recombinant protein by affinity chromatography, no difference in activity compared to the wild type | Haemophilus influenzae |
of the recombinant protein by affinity chromatography, no difference in activity compared to the wild type | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.7 | - |
at 2.0 mM ATP, without GTP | Enterococcus faecalis |
2.7 | - |
at 2.0 mM ATP, without GTP | Bacillus subtilis |
3.7 | - |
at 2.0 mM ATP, without GTP | Streptococcus pneumoniae |
4.6 | - |
at 2.0 mM ATP, without GTP | Staphylococcus aureus |
5.1 | - |
activity dependent of concentrations of ATP, at 2.0 mM ATP, without GTP | Neisseria meningitidis |
9.1 | - |
activity independent of concentrations of ATP, at 2.0 mM ATP, without GTP | Haemophilus influenzae |
9.1 | - |
at 8.0 mM ATP, without GTP | Enterococcus faecalis |
9.7 | - |
at 2.0 mM ATP, with 0.5 mM GTP | Enterococcus faecalis |
10.2 | - |
at 8.0 mM ATP, with 0.5 mM GTP | Neisseria meningitidis |
11.3 | - |
at 8.0 mM ATP, without GTP | Neisseria meningitidis |
11.8 | - |
at 8.0 mM ATP, without GTP | Haemophilus influenzae |
12.8 | - |
at 8.0 mM ATP, without GTP | Staphylococcus aureus |
18.1 | - |
at 8.0 mM ATP, without GTP | Bacillus subtilis |
19.4 | - |
at 8.0 mM ATP, with 0.5 mM GTP | Enterococcus faecalis |
23.5 | - |
at 2.0 mM ATP, with 0.5 mM GTP | Staphylococcus aureus |
23.6 | - |
at 8.0 mM ATP, with 0.5 mM GTP | Staphylococcus aureus |
26.1 | - |
at 2.0 mM ATP, without GTP | Escherichia coli |
28.1 | - |
at 8.0 mM ATP, without GTP | Escherichia coli |
28.6 | - |
at 2.0 mM ATP, with 0.5 mM GTP | Bacillus subtilis |
38.9 | - |
at 8.0 mM ATP, without GTP | Streptococcus pneumoniae |
39.1 | - |
at 8.0 mM ATP, with 0.5 mM GTP | Bacillus subtilis |
46.8 | - |
activity independent of concentrations of ATP, at 2.0 mM ATP, without GTP | Salmonella enterica subsp. enterica serovar Typhimurium |
47.5 | - |
at 8.0 mM ATP, without GTP | Salmonella enterica subsp. enterica serovar Typhimurium |
52.8 | - |
at 2.0 mM ATP, with 0.5 mM GTP | Haemophilus influenzae |
57.7 | - |
at 2.0 mM ATP, with 0.5 mM GTP | Neisseria meningitidis |
60.5 | - |
at 8.0 mM ATP, with 0.5 mM GTP | Haemophilus influenzae |
65.9 | - |
at 2.0 mM ATP, with 0.5 mM GTP | Escherichia coli |
68.5 | - |
at 2.0 mM ATP, with 0.5 mM GTP | Streptococcus pneumoniae |
71.1 | - |
at 8.0 mM ATP, with 0.5 mM GTP | Escherichia coli |
87.4 | - |
at 8.0 mM ATP, with 0.5 mM GTP | Streptococcus pneumoniae |
124.2 | - |
at 8.0 mM ATP, with 0.5 mM GTP | Salmonella enterica subsp. enterica serovar Typhimurium |
134.3 | - |
at 2.0 mM ATP, with 0.5 mM GTP | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + UDP | - |
r | |
ATP + UMP | - |
Staphylococcus aureus | ADP + UDP | - |
r | |
ATP + UMP | - |
Haemophilus influenzae | ADP + UDP | - |
r | |
ATP + UMP | - |
Enterococcus faecalis | ADP + UDP | - |
r | |
ATP + UMP | - |
Streptococcus pneumoniae | ADP + UDP | - |
r | |
ATP + UMP | - |
Bacillus subtilis | ADP + UDP | - |
r | |
ATP + UMP | - |
Escherichia coli | ADP + UDP | - |
r | |
ATP + UMP | - |
Neisseria meningitidis | ADP + UDP | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | gel filtration | Staphylococcus aureus |
hexamer | gel filtration | Enterococcus faecalis |
hexamer | gel filtration | Streptococcus pneumoniae |
hexamer | gel filtration | Bacillus subtilis |
hexamer | gel filtration | Neisseria meningitidis |
hexamer | gel permeation | Salmonella enterica subsp. enterica serovar Typhimurium |
hexamer | gel permeation | Haemophilus influenzae |
hexamer | gel permeation | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ATP:UMP phosphotransferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
ATP:UMP phosphotransferase | - |
Staphylococcus aureus |
ATP:UMP phosphotransferase | - |
Haemophilus influenzae |
ATP:UMP phosphotransferase | - |
Enterococcus faecalis |
ATP:UMP phosphotransferase | - |
Streptococcus pneumoniae |
ATP:UMP phosphotransferase | - |
Bacillus subtilis |
ATP:UMP phosphotransferase | - |
Escherichia coli |
ATP:UMP phosphotransferase | - |
Neisseria meningitidis |
UMP kinase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
UMP kinase | - |
Staphylococcus aureus |
UMP kinase | - |
Haemophilus influenzae |
UMP kinase | - |
Enterococcus faecalis |
UMP kinase | - |
Streptococcus pneumoniae |
UMP kinase | - |
Bacillus subtilis |
UMP kinase | - |
Escherichia coli |
UMP kinase | - |
Neisseria meningitidis |
uridine monophosphate kinase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
uridine monophosphate kinase | - |
Staphylococcus aureus |
uridine monophosphate kinase | - |
Haemophilus influenzae |
uridine monophosphate kinase | - |
Enterococcus faecalis |
uridine monophosphate kinase | - |
Streptococcus pneumoniae |
uridine monophosphate kinase | - |
Bacillus subtilis |
uridine monophosphate kinase | - |
Neisseria meningitidis |
uridylate kinase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
uridylate kinase | - |
Staphylococcus aureus |
uridylate kinase | - |
Haemophilus influenzae |
uridylate kinase | - |
Enterococcus faecalis |
uridylate kinase | - |
Streptococcus pneumoniae |
uridylate kinase | - |
Bacillus subtilis |
uridylate kinase | - |
Neisseria meningitidis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
UMP | at 2.0 mM ATP, without GTP | Neisseria meningitidis | |
0.17 | - |
UMP | at 12 mM ATP, without GTP | Haemophilus influenzae | |
0.23 | - |
UMP | at 10 mM ATP, without GTP | Neisseria meningitidis | |
0.31 | - |
UMP | at 1.0 mM ATP, without GTP | Haemophilus influenzae | |
0.44 | - |
UMP | at 2.0 mM ATP, without GTP | Escherichia coli | |
0.6 | - |
UMP | at 2.0 mM ATP, without GTP | Escherichia coli | |
0.66 | - |
UMP | at 2.0 mM ATP, without GTP, no inhibition at 30 mM ATP | Bacillus subtilis | |
4.4 | - |
UMP | at 2.0 mM ATP, without GTP | Streptococcus pneumoniae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
at 0.05 mM UMP, UMP protects for inhibition by Mg2+ free UTP | Neisseria meningitidis | UTP | |
0.13 | - |
at 0.2 mM UMP, UMP protects for inhibition by Mg2+ free UTP | Neisseria meningitidis | UTP |