Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | - |
Escherichia coli |
Application | Comment | Organism |
---|---|---|
drug development | as bacterial UMP kinases have no counterpart in eukaryotes, the information provided here can help the design of new antibiotics | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
bound to the UMP substrate, resolved at 2.3 A resolution, bound to the UDP product, resolved at 2.6 A resolution, bound to UTP, resolved at 2.45 A resolution | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
N140A | decreases half-denaturation temperature of UMP kinase by around 10°C, moderate loss of sensitivity to UTP inhibition, important loss in activation by GTP | Escherichia coli |
T138A | decreases half-denaturation temperature of UMP kinase by around 10°C, results in 4times higher Km for UMP, moderate loss of sensitivity to UTP inhibition, important loss in activation by GTP | Escherichia coli |
T138A/N140A | decreases half-denaturation temperature of UMP kinase by around 25°C, increases the apparant Km for ATP and UMP by a factor of 2.6 and 12, respectively | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UTP | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
UMP | wild-type | Escherichia coli | |
0.056 | - |
UMP | mutant N140A | Escherichia coli | |
0.083 | - |
ATP | mutant N140A | Escherichia coli | |
0.16 | - |
ATP | wild-type | Escherichia coli | |
0.19 | - |
UMP | mutant T138A | Escherichia coli | |
0.29 | - |
ATP | mutant T138A | Escherichia coli | |
0.42 | - |
ATP | mutant T138A/N140A | Escherichia coli | |
0.6 | - |
UMP | mutant T138A/N140A | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | Escherichia coli | - |
ADP + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A7E9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
52 | - |
mutant N140A | Escherichia coli |
88 | - |
mutant T138A | Escherichia coli |
90 | - |
mutant T138A/N140A | Escherichia coli |
96 | - |
wild-type | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | - |
Escherichia coli | ADP + UDP | - |
? | |
ATP + UMP | at 30°C, pH 7.4, in the presence of 2 mM MgCl2 | Escherichia coli | ADP + UDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | crystal structure | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
UMP kinase | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
mutant T138A/N140A, temperature of half-inactivation | Escherichia coli |
56 | - |
mutant T138A, temperature of half-inactivation | Escherichia coli |
59 | - |
mutant N140A, temperature of half-inactivation | Escherichia coli |
68 | - |
wild-type, temperature of half-inactivation | Escherichia coli |