Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3)/pDIA17 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D174N | the enzyme is not recognized by a monoclonal antibody | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | near the bacterial membranes | Escherichia coli | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 26000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | Escherichia coli | - |
ADP + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A7E9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl (pH 7.4) as insoluble proteins, after solubilization with 0.1 M borate (pH 9) or with 1 mM UTP in 50 mM Tris-HCl (pH 7.4), the enzyme is fully active | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | - |
Escherichia coli | ADP + UDP | - |
? | |
ATP + UMP | 30°C, pH 7.4 | Escherichia coli | ADP + UDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
UMP kinase | - |
Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | - |
7.24 |