Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | most effective at pH 8 and a high (1 mM) UMP concentration | Escherichia coli |
Application | Comment | Organism |
---|---|---|
drug development | potential target for antibacterial drugs | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D146N | 84% of wild-type activity in the pellet of the sonicated bacterial extract | Escherichia coli |
D159N | exhibits higher solubility than the wild-type protein at neutral pH, 10% of wild-type activity in the pellet of the sonicated bacterial extract | Escherichia coli |
D168N | 97% of wild-type activity in the pellet of the sonicated bacterial extract | Escherichia coli |
D174N | loss of two-thirds of its activity after 3 months storage at 4°C in 50 mM Tris-HCl (pH 7.4), 98% of wild-type activity in the pellet of the sonicated bacterial extract | Escherichia coli |
D201N | exhibits higher solubility than the wild-type protein at neutral pH, 42% of wild-type activity in the pellet of the sonicated bacterial extract | Escherichia coli |
D77N | 84% of wild-type activity in the pellet of the sonicated bacterial extract, insensitive to activation by GTP | Escherichia coli |
R62H | 83% of wild-type activity in the pellet of the sonicated bacterial extract, insensitive to activation by GTP | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | mutant D201N is inhibited by GTP above 0.2 mM | Escherichia coli | |
UTP | the inhibitory effect is maximal at pH 8 and a low (0.1 mM) UMP concentration | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
ATP | mutant D146N, at pH 6 | Escherichia coli | |
0.043 | - |
UMP | wild-type, at pH 8 | Escherichia coli | |
0.048 | - |
ATP | wild-type, at pH 6 | Escherichia coli | |
0.052 | - |
UMP | mutant D159N, at pH 8 | Escherichia coli | |
0.059 | - |
UMP | mutant D146N, at pH 8 | Escherichia coli | |
0.06 | - |
UMP | mutant D168N, at pH 8 | Escherichia coli | |
0.095 | - |
ATP | mutant D159N, at pH 6 | Escherichia coli | |
0.1 | - |
ATP | mutant D146N, at pH 8 | Escherichia coli | |
0.12 | - |
ATP | mutant D77N, at pH 6 | Escherichia coli | |
0.12 | - |
ATP | wild-type, at pH 8 | Escherichia coli | |
0.14 | - |
ATP | mutant D168N, at pH 6 | Escherichia coli | |
0.17 | - |
ATP | mutant D174N, at pH 6 | Escherichia coli | |
0.17 | - |
UMP | wild-type, at pH 6 | Escherichia coli | |
0.2 | - |
ATP | mutant D201N, at pH 6 | Escherichia coli | |
0.22 | - |
UMP | mutant D201N, at pH 8 | Escherichia coli | |
0.24 | - |
ATP | mutant R62H, at pH 6 | Escherichia coli | |
0.27 | - |
UMP | mutant D159N, at pH 6 | Escherichia coli | |
0.29 | - |
ATP | mutant D159N, at pH 8 | Escherichia coli | |
0.31 | - |
ATP | mutant D174N, at pH 8 | Escherichia coli | |
0.31 | - |
UMP | mutant D174N, at pH 8 | Escherichia coli | |
0.32 | - |
UMP | mutant D168N, at pH 6 | Escherichia coli | |
0.32 | - |
UMP | mutant R62H, at pH 8 | Escherichia coli | |
0.42 | - |
ATP | mutant D168N, at pH 8 | Escherichia coli | |
0.45 | - |
UMP | mutant D146N, at pH 6 | Escherichia coli | |
0.46 | - |
ATP | mutant D201N, at pH 8 | Escherichia coli | |
0.47 | - |
UMP | mutant D77N, at pH 8 | Escherichia coli | |
0.48 | - |
ATP | mutant D77N, at pH 8 | Escherichia coli | |
0.86 | - |
UMP | mutant D201N, at pH 6 | Escherichia coli | |
1.26 | - |
UMP | mutant R62H, at pH 6 | Escherichia coli | |
1.78 | - |
UMP | mutant D77N, at pH 6 | Escherichia coli | |
3 | - |
ATP | mutant R62H, at pH 8 | Escherichia coli | |
3.7 | - |
UMP | mutant D174N, at pH 6 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | Escherichia coli | - |
ADP + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A7E9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.15 | - |
mutant D146N, at pH 6 | Escherichia coli |
0.32 | - |
mutant D146N, at pH 8 | Escherichia coli |
1.04 | - |
mutant D201N, at pH 6 | Escherichia coli |
1.5 | - |
mutant R62H, at pH 6 | Escherichia coli |
1.9 | - |
mutant D77N, at pH 6 | Escherichia coli |
4.4 | - |
mutant R62H, at pH 8 | Escherichia coli |
8.2 | - |
mutant D201N, at pH 8 | Escherichia coli |
8.2 | - |
mutant D77N, at pH 8 | Escherichia coli |
32 | - |
mutant D174N, at pH 6 | Escherichia coli |
45 | - |
mutant D174N, at pH 8 | Escherichia coli |
63 | - |
mutant D168N, at pH 6 | Escherichia coli |
86.6 | - |
mutant D168N, at pH 8 | Escherichia coli |
105 | - |
wild-type, at pH 6 | Escherichia coli |
128 | - |
mutant D159N, at pH 6 | Escherichia coli |
128 | - |
wild-type, at pH 8 | Escherichia coli |
153 | - |
mutant D159N, at pH 8 | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl (pH 7.4), several months, no significant loss of activity. An exception is mutant D174N, which loses two-thirds of its activity after 3 months at 4°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | - |
Escherichia coli | ADP + UDP | - |
? | |
ATP + UMP | 30°C, pH 6, 7.4 or 8, 2 mM MgCl2 | Escherichia coli | ADP + UDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha6, gel filtration | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
UMP kinase | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
Tm for mutant D201N | Escherichia coli |
52 | - |
Tm for mutant D77N | Escherichia coli |
59 | 62 | mutants R62H, D146N, D168N and D174N are half inactivated between 59 and 62°C | Escherichia coli |
63 | - |
wild-type Tm | Escherichia coli |
73 | - |
Tm for mutant D159N | Escherichia coli |