Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3'-anthraniloyl-2'-deoxyguanosine-5'-triphosphate | - |
Bacillus subtilis | |
dGTP | - |
Bacillus subtilis | |
GTP | half-maximum activation is reached at 0.1 mM GTP, in the absence of GTP the enzyme exhibits less than 10% of its maximum activity | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
expression in Escherichia coli strain BL21(DE3)/pDIA17 | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UTP | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
UMP | in the absence of GTP | Bacillus subtilis | |
0.03 | - |
UMP | in the presence of GTP | Bacillus subtilis | |
0.05 | - |
UMP | - |
Escherichia coli | |
0.11 | - |
5-fluoro-UMP | - |
Escherichia coli | |
0.12 | - |
5-fluoro-UMP | - |
Bacillus subtilis | |
0.14 | - |
6-aza-UMP | - |
Bacillus subtilis | |
0.71 | - |
6-aza-UMP | - |
Escherichia coli | |
0.9 | - |
ATP | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26080 | - |
monomer, calculated from amino acid sequence | Bacillus subtilis |
26080 | - |
monomer, mass spectroscopy | Bacillus subtilis |
28120 | - |
His-tagged protein, monomer, mass spectroscopy | Bacillus subtilis |
28250 | - |
His-tagged protein, monomer, calculated from amino acid sequence | Bacillus subtilis |
154000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | Escherichia coli | - |
ADP + UDP | - |
? | |
ATP + UMP | Bacillus subtilis | - |
ADP + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O31749 | - |
- |
Escherichia coli | P0A7E9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | - |
with 6-aza-UMP as substrate | Bacillus subtilis |
24 | - |
with 5-fluoro-UMP as substrate | Bacillus subtilis |
25 | - |
His-tagged protein | Bacillus subtilis |
26 | - |
native protein | Bacillus subtilis |
67 | - |
with 6-aza-UMP as substrate | Escherichia coli |
126 | - |
with UMP as substrate | Escherichia coli |
162 | - |
with 5-fluoro-UMP as substrate | Escherichia coli |
Storage Stability | Organism |
---|---|
room temperature, 50 mM Tris-HCl (pH 7.4), 0.1 M NaCl, 2 mM UTP, 2 weeks, no loss of activity | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-fluoro-UMP | - |
Escherichia coli | ADP + 5-fluoro-UDP | - |
? | |
ATP + 5-fluoro-UMP | - |
Bacillus subtilis | ADP + 5-fluoro-UDP | - |
? | |
ATP + 6-aza-UMP | - |
Escherichia coli | ADP + 6-aza-UDP | - |
? | |
ATP + 6-aza-UMP | - |
Bacillus subtilis | ADP + 6-aza-UDP | - |
? | |
ATP + UMP | - |
Escherichia coli | ADP + UDP | - |
? | |
ATP + UMP | - |
Bacillus subtilis | ADP + UDP | - |
? | |
ATP + UMP | pH 7.4, 2 mM MgCl2, 30°C | Escherichia coli | ADP + UDP | - |
? | |
ATP + UMP | pH 7.4, 2 mM MgCl2, 30°C | Bacillus subtilis | ADP + UDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha6, gel filtration | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
UMP kinase | - |
Escherichia coli |
UMP kinase | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
half-maximal inactivation | Bacillus subtilis |
70 | - |
half-maximal inactivation in the presence of 1 mM UTP | Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
UTP | - |
Bacillus subtilis |