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Literature summary for 2.7.4.22 extracted from

  • Serina, L.; Bucurenci, N.; Gilles, A.M.; Surewicz, W.K.; Fabian, H.; Mantsch, H.H.; Takahashi, M.; Petrescu, I.; Batelier, G.; Barzu, O.
    Structural properties of UMP-kinase from Escherichia coli: modulation of protein solubility by pH and UTP (1996), Biochemistry, 35, 7003-7011.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
GTP
-
Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
D159N appears at neutral pH almost exclusively as a hexamer Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
UTP
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
UMP wild-type, at pH 7.4 Escherichia coli
0.048
-
ATP wild-type, at pH 6.0 Escherichia coli
0.052
-
UMP mutant D159N, at pH 7.4 Escherichia coli
0.095
-
ATP mutant D159N, at pH 6.0 Escherichia coli
0.12
-
ATP wild-type, at pH 7.4 Escherichia coli
0.17
-
UMP wild-type, at pH 6.0 Escherichia coli
0.27
-
UMP mutant D159N, at pH 6.0 Escherichia coli
0.286
-
ATP mutant D159N, at pH 7.4 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25800
-
monomer, SDS-PAGE Escherichia coli
32100
-
mutant D159N, monomer, sedimentation velocity at pH 9.0 Escherichia coli
32800
-
wild-type, monomer, sedimentation velocity at pH 9.0 Escherichia coli
35300
-
mutant D159N, monomer, sedimentation velocity at pH 7.4 Escherichia coli
59400
-
wild-type, dimer, sedimentation velocity at pH 6.0 Escherichia coli
145300
-
wild-type, hexamer, sedimentation velocity at pH 9.0 Escherichia coli
146400
-
mutant D159N, hexamer, sedimentation velocity at pH 9.0 Escherichia coli
147400
-
mutant D159N, hexamer, sedimentation velocity at pH 7.4 Escherichia coli
150000
-
gel filtration Escherichia coli
157000
-
wild-type, hexamer, sedimentation velocity at pH 6.0 Escherichia coli
297400
-
wild-type, oligomer, sedimentation velocity at pH 9.0 Escherichia coli
482900
-
wild-type, oligomer, sedimentation velocity at pH 6.0 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + UMP Escherichia coli
-
ADP + UDP
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A7E9
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant D159N Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
105
-
wild-type, at pH 6.0 Escherichia coli
128
-
mutant D159N, at pH 6.0 Escherichia coli
128
-
wild-type, at pH 7.4 Escherichia coli
153
-
mutant D159N, at pH 7.4 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UMP
-
Escherichia coli ADP + UDP
-
?
ATP + UMP 30°C, pH 7.4, 5 mM MgCl2 Escherichia coli ADP + UDP
-
?

Subunits

Subunits Comment Organism
hexamer alpha6, gel filtration Escherichia coli

Synonyms

Synonyms Comment Organism
UMP-kinase
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
61.6
-
Tm Escherichia coli
62.7
-
Tm in the presence of 0.1 mM GTP Escherichia coli
63.7
-
Tm in the presence of 1 mM ATP Escherichia coli
65.8
-
Tm in the presence of 1 mM GTP Escherichia coli
69.8
-
Tm in the presence of 5 mM GTP Escherichia coli
74.6
-
Tm in the presence of 0.1 mM UTP Escherichia coli
75.6
-
Tm in the presence of 1 mM UMP Escherichia coli
82.4
-
Tm in the presence of 1 mM UTP Escherichia coli
86.5
-
Tm in the presence of 5 mM UTP Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10
-
Escherichia coli