Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | - |
Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D159N | appears at neutral pH almost exclusively as a hexamer | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UTP | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
UMP | wild-type, at pH 7.4 | Escherichia coli | |
0.048 | - |
ATP | wild-type, at pH 6.0 | Escherichia coli | |
0.052 | - |
UMP | mutant D159N, at pH 7.4 | Escherichia coli | |
0.095 | - |
ATP | mutant D159N, at pH 6.0 | Escherichia coli | |
0.12 | - |
ATP | wild-type, at pH 7.4 | Escherichia coli | |
0.17 | - |
UMP | wild-type, at pH 6.0 | Escherichia coli | |
0.27 | - |
UMP | mutant D159N, at pH 6.0 | Escherichia coli | |
0.286 | - |
ATP | mutant D159N, at pH 7.4 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25800 | - |
monomer, SDS-PAGE | Escherichia coli |
32100 | - |
mutant D159N, monomer, sedimentation velocity at pH 9.0 | Escherichia coli |
32800 | - |
wild-type, monomer, sedimentation velocity at pH 9.0 | Escherichia coli |
35300 | - |
mutant D159N, monomer, sedimentation velocity at pH 7.4 | Escherichia coli |
59400 | - |
wild-type, dimer, sedimentation velocity at pH 6.0 | Escherichia coli |
145300 | - |
wild-type, hexamer, sedimentation velocity at pH 9.0 | Escherichia coli |
146400 | - |
mutant D159N, hexamer, sedimentation velocity at pH 9.0 | Escherichia coli |
147400 | - |
mutant D159N, hexamer, sedimentation velocity at pH 7.4 | Escherichia coli |
150000 | - |
gel filtration | Escherichia coli |
157000 | - |
wild-type, hexamer, sedimentation velocity at pH 6.0 | Escherichia coli |
297400 | - |
wild-type, oligomer, sedimentation velocity at pH 9.0 | Escherichia coli |
482900 | - |
wild-type, oligomer, sedimentation velocity at pH 6.0 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | Escherichia coli | - |
ADP + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A7E9 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant D159N | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
105 | - |
wild-type, at pH 6.0 | Escherichia coli |
128 | - |
mutant D159N, at pH 6.0 | Escherichia coli |
128 | - |
wild-type, at pH 7.4 | Escherichia coli |
153 | - |
mutant D159N, at pH 7.4 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | - |
Escherichia coli | ADP + UDP | - |
? | |
ATP + UMP | 30°C, pH 7.4, 5 mM MgCl2 | Escherichia coli | ADP + UDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha6, gel filtration | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
UMP-kinase | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
61.6 | - |
Tm | Escherichia coli |
62.7 | - |
Tm in the presence of 0.1 mM GTP | Escherichia coli |
63.7 | - |
Tm in the presence of 1 mM ATP | Escherichia coli |
65.8 | - |
Tm in the presence of 1 mM GTP | Escherichia coli |
69.8 | - |
Tm in the presence of 5 mM GTP | Escherichia coli |
74.6 | - |
Tm in the presence of 0.1 mM UTP | Escherichia coli |
75.6 | - |
Tm in the presence of 1 mM UMP | Escherichia coli |
82.4 | - |
Tm in the presence of 1 mM UTP | Escherichia coli |
86.5 | - |
Tm in the presence of 5 mM UTP | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | - |
Escherichia coli |