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Literature summary for 2.7.4.22 extracted from

  • Serina, L.; Blondin, C.; Krin, E.; Sismeiro, O.; Danchin, A.; Sakamoto, H.; Gilles, A.M.; Barzu, O.
    Escherichia coli UMP-kinase, a member of the aspartokinase family, is a hexamer regulated by guanine nucleotides and UTP (1995), Biochemistry, 34, 5066-5074.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cGMP lower affinity or extent of activation than GTP Escherichia coli
dGTP lower affinity or extent of activation than GTP Escherichia coli
dialdehyde-GTP slight stimulatory effect, 30% maximal increase in UMP-kinase activity Escherichia coli
GMP lower affinity or extent of activation than GTP Escherichia coli
GTP activates only the wild-type, not the mutant D201N Escherichia coli
guanosine lower affinity or extent of activation than GTP Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
overproduced in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
D201N exhibits 10% of the wild-type activity, has altered stability and regulatory properties Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
GTP mutant D201N Escherichia coli
guanylyl imidodiphosphate mutant D201N Escherichia coli
additional information no inhibition by dUMP, CMP, dCMP or TMP Escherichia coli
UTP
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
UMP at 1 mM ATP, wild-type Escherichia coli
0.11
-
UMP mutant D201N Escherichia coli
0.12
-
ATP at 1 mM UMP, wild-type Escherichia coli
0.32
-
ATP mutant D201N Escherichia coli
1
-
GTP wild-type Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25800
-
monomer, calculated from amino acid sequence Escherichia coli
156000
-
sedimentation equilibrium Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + UMP Escherichia coli
-
ADP + UDP
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A7E9
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
mutant D201N, rate of the reverse reaction Escherichia coli
3.6
-
mutant D201N, in the presence of 1 mM ATP and 1 mM UMP Escherichia coli
160
-
wild-type, in the presence of ATP and GTP Escherichia coli

Storage Stability

Storage Stability Organism
room temperature, 0.1 M borate buffer (pH 8.5), 2 months, no loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UMP
-
Escherichia coli ADP + UDP
-
r
ATP + UMP 30°C, pH 7.4, 2 mM MgCl2 Escherichia coli ADP + UDP
-
r
GTP + UMP reaction rate is 3-5% of that with ATP Escherichia coli GDP + UDP
-
r
additional information no reaction with dUMP, CMP, dCMP or TMP as substrate Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
hexamer alpha6, sedimentation equilibrium Escherichia coli

Synonyms

Synonyms Comment Organism
UMP-kinase
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
51
-
mutant D201N is half-inactivated Escherichia coli
64
-
wild-type protein is half-inactivated Escherichia coli