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Literature summary for 2.7.4.22 extracted from

  • Fassy, F.; Krebs, O.; Lowinski, M.; Ferrari, P.; Winter, J.; Collard-Dutilleul, V.; Salahbey Hocini, K.
    UMP kinase from Streptococcus pneumoniae: evidence for co-operative ATP binding and allosteric regulation (2004), Biochem. J., 384, 619-627.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
GTP increases the affinity for ATP Streptococcus pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
GTP at high concentrations Streptococcus pneumoniae
UTP decreases the affinity for ATP Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
UMP in the presence of MgCl2 Streptococcus pneumoniae
0.15
-
UMP in the presence of MnCl2 Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 in the presence of MgCl2 the saturation kinetics of recombinant purified UMP kinase are hyperbolic for UMP and sigmoidal for ATP Streptococcus pneumoniae
MnCl2 in the presence of MnCl2 the saturation kinetics of recombinant purified UMP kinase are hyperbolic for UMP and sigmoidal for ATP Streptococcus pneumoniae
additional information UMP kinase is totally inactive in the absence of MgCl2 or MnCl2 Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26300
-
mass spectroscopy, N-terminal sequencing Streptococcus pneumoniae
87500
-
minor form, gel filtration Streptococcus pneumoniae
189000
-
major form, representing two-thirds of the protein population, gel filtration Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + UMP Streptococcus pneumoniae
-
ADP + UDP
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q97R83
-
-

Purification (Commentary)

Purification (Comment) Organism
the recombinant enzyme from Escherichia coli Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
GTP and UTP are extremely poor substrates with an activity below 1 micromol/min/mg with MnCl2 or MgCl2 Streptococcus pneumoniae
66
-
with MgCl2 and ATP as phosphate donor Streptococcus pneumoniae
130
-
with MnCl2 and ATP as phosphate donor Streptococcus pneumoniae
135
-
at pH 6.5, in the presence of MnCl2 Streptococcus pneumoniae
182
-
at pH 6.5, in the presence of MgCl2 Streptococcus pneumoniae
274
-
at pH 7.5, in the presence of MnCl2 Streptococcus pneumoniae
399
-
at pH 7.5, in the presence of MgCl2 Streptococcus pneumoniae
666
-
at pH 8.5, in the presence of MnCl2 Streptococcus pneumoniae

Storage Stability

Storage Stability Organism
-80°C, 12 months storage, less than 10% loss of activity Streptococcus pneumoniae
0°C, 6 h, less than 10% loss of activity Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UMP
-
Streptococcus pneumoniae ADP + UDP
-
?
ATP + UMP at 37°C, pH 7.5, in the presence of MnCl2 or MgCl2 Streptococcus pneumoniae ADP + UDP
-
?

Subunits

Subunits Comment Organism
More calculations indicate a heptamer of the major form and a trimer of the minor one. Homoheptamers are not frequently observed and the major form may, rather, be a hexamer Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
UMP kinase
-
Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
120
-
ATP in the presence of MnCl2 Streptococcus pneumoniae
175
-
ATP in the presence of MgCl2 Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
below 6.5 with 2 mM ATP, in the presence of MgCl2 Streptococcus pneumoniae
additional information
-
below 6.5 with 2 mM ATP, in the presence of MnCl2 Streptococcus pneumoniae
6.5
-
with 4 mM ATP, in the presence of MgCl2 Streptococcus pneumoniae
7.1
-
with 4 mM ATP, in the presence of MnCl2 Streptococcus pneumoniae
7.4
-
with 10 mM ATP, in the presence of MgCl2 Streptococcus pneumoniae
7.8
-
with 10 mM ATP, in the presence of MnCl2 Streptococcus pneumoniae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.14
-
GTP in the presence of MnCl2 Streptococcus pneumoniae
0.21
-
GTP in the presence of MgCl2 Streptococcus pneumoniae
0.21
-
UTP in the presence of MnCl2 Streptococcus pneumoniae
0.28
-
UTP in the presence of MgCl2 Streptococcus pneumoniae