Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | increases the affinity for ATP | Streptococcus pneumoniae |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | at high concentrations | Streptococcus pneumoniae | |
UTP | decreases the affinity for ATP | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
UMP | in the presence of MgCl2 | Streptococcus pneumoniae | |
0.15 | - |
UMP | in the presence of MnCl2 | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | in the presence of MgCl2 the saturation kinetics of recombinant purified UMP kinase are hyperbolic for UMP and sigmoidal for ATP | Streptococcus pneumoniae | |
MnCl2 | in the presence of MnCl2 the saturation kinetics of recombinant purified UMP kinase are hyperbolic for UMP and sigmoidal for ATP | Streptococcus pneumoniae | |
additional information | UMP kinase is totally inactive in the absence of MgCl2 or MnCl2 | Streptococcus pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26300 | - |
mass spectroscopy, N-terminal sequencing | Streptococcus pneumoniae |
87500 | - |
minor form, gel filtration | Streptococcus pneumoniae |
189000 | - |
major form, representing two-thirds of the protein population, gel filtration | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | Streptococcus pneumoniae | - |
ADP + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | Q97R83 | - |
- |
Purification (Comment) | Organism |
---|---|
the recombinant enzyme from Escherichia coli | Streptococcus pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
GTP and UTP are extremely poor substrates with an activity below 1 micromol/min/mg with MnCl2 or MgCl2 | Streptococcus pneumoniae |
66 | - |
with MgCl2 and ATP as phosphate donor | Streptococcus pneumoniae |
130 | - |
with MnCl2 and ATP as phosphate donor | Streptococcus pneumoniae |
135 | - |
at pH 6.5, in the presence of MnCl2 | Streptococcus pneumoniae |
182 | - |
at pH 6.5, in the presence of MgCl2 | Streptococcus pneumoniae |
274 | - |
at pH 7.5, in the presence of MnCl2 | Streptococcus pneumoniae |
399 | - |
at pH 7.5, in the presence of MgCl2 | Streptococcus pneumoniae |
666 | - |
at pH 8.5, in the presence of MnCl2 | Streptococcus pneumoniae |
Storage Stability | Organism |
---|---|
-80°C, 12 months storage, less than 10% loss of activity | Streptococcus pneumoniae |
0°C, 6 h, less than 10% loss of activity | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | - |
Streptococcus pneumoniae | ADP + UDP | - |
? | |
ATP + UMP | at 37°C, pH 7.5, in the presence of MnCl2 or MgCl2 | Streptococcus pneumoniae | ADP + UDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | calculations indicate a heptamer of the major form and a trimer of the minor one. Homoheptamers are not frequently observed and the major form may, rather, be a hexamer | Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
UMP kinase | - |
Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
120 | - |
ATP | in the presence of MnCl2 | Streptococcus pneumoniae | |
175 | - |
ATP | in the presence of MgCl2 | Streptococcus pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
below 6.5 with 2 mM ATP, in the presence of MgCl2 | Streptococcus pneumoniae |
additional information | - |
below 6.5 with 2 mM ATP, in the presence of MnCl2 | Streptococcus pneumoniae |
6.5 | - |
with 4 mM ATP, in the presence of MgCl2 | Streptococcus pneumoniae |
7.1 | - |
with 4 mM ATP, in the presence of MnCl2 | Streptococcus pneumoniae |
7.4 | - |
with 10 mM ATP, in the presence of MgCl2 | Streptococcus pneumoniae |
7.8 | - |
with 10 mM ATP, in the presence of MnCl2 | Streptococcus pneumoniae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
GTP | in the presence of MnCl2 | Streptococcus pneumoniae | |
0.21 | - |
GTP | in the presence of MgCl2 | Streptococcus pneumoniae | |
0.21 | - |
UTP | in the presence of MnCl2 | Streptococcus pneumoniae | |
0.28 | - |
UTP | in the presence of MgCl2 | Streptococcus pneumoniae |