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Literature summary for 2.7.4.21 extracted from

  • Zhan, H.; Zhong, Y.; Yang, Z.; Xia, H.
    Enzyme activities of Arabidopsis inositol polyphosphate kinases AtIPK2? and AtIPK2? are involved in pollen development, pollen tube guidance and embryogenesis. (2015), Plant J., 82, 758-771.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene IMPKb Arabidopsis thaliana
gene IPMKa, Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of a atipk2alpha/atipk2beta double mutant with male gametophyte and embryogenesis defects, the mutant cannot be rescued by either catalytically inactive or substrate specificity-altered variants of AtIPK2beta. The single knock-out mutant of atipk2alpha is indistinguishable from the wild-type. Transmission analysis of atipk2alpha and atipk2beta mutant alleles and Segregation analysis of atipk2alpha and atipk2beta mutants, overview. Complementation analysis of the atipk2alpha/atipk2beta double mutant, development-defective phenotype Arabidopsis thaliana
additional information construction of a atipk2alpha/atipk2beta double mutant with male gametophyte and embryogenesis defects, the mutant cannot be rescued by either catalytically inactive or substrate specificity-altered variants of AtIPK2beta. The single knock-out mutant of atipk2beta is indistinguishable from the wild-type. Transmission analysis of atipk2alpha and atipk2beta mutant alleles and Segregation analysis of atipk2alpha and atipk2beta mutants, overview. Complementation analysis of the atipk2alpha/atipk2beta double mutant, development-defective phenotype Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1-diphosphate pentakisphosphate Arabidopsis thaliana
-
ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate
-
?
ATP + 1D-myo-inositol hexakisphosphate Arabidopsis thaliana
-
ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FLT2
-
-
Arabidopsis thaliana Q9LY23
-
-

Source Tissue

Source Tissue Comment Organism Textmining
gametophyte male, isozymes AtIPK2alpha and AtIPK2beta have overlapping expression in male gametophyte Arabidopsis thaliana
-
additional information in vitro and in vivo analysis of pollen germination and pollen tube growth of wild-type and mutant strains, detection of pollen tube guidance in cross-pollination, overview Arabidopsis thaliana
-
pollen
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1-diphosphate pentakisphosphate
-
Arabidopsis thaliana ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate
-
?
ATP + 1D-myo-inositol hexakisphosphate
-
Arabidopsis thaliana ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate
-
?
additional information the consensus PxxxDxKxG motif is responsible for substrate binding and is highly conserved in all known IPK2s Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
AtIPK2alpha
-
Arabidopsis thaliana
AtIpk2beta
-
Arabidopsis thaliana
inositol polyphosphate kinase
-
Arabidopsis thaliana
Ipk2
-
Arabidopsis thaliana
IPMK
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction expressing either catalytically inactive or substrate specificity-altered variants of AtIPK2beta cannot rescue the male gametophyte and embryogenesis defects of the atipk2alpha/atipk2beta double mutant. The mutation of AtIPK2alpha and AtIPK2beta results in severely reduced transmission of male gametophyte as a result of abnormal pollen development and defective pollen tube guidance. Overexpression or reduction of the expression of AtIPK2alpha and AtIPK2beta reveal their roles in auxiliary shoot branching, abiotic stress responses and root growth. Phenotype, detailed overview Arabidopsis thaliana
physiological function the enzyme is a key component for inositol polyphosphate turnover. The kinase activity of AtIPK2 is required for pollen development, pollen tube guidance and embryogenesis, requirement of inositol polyphosphate signaling in plant sexual reproduction. Isozymes AtIPK2alpha and AtIPK2beta act redundantly during pollen development, pollen tube guidance and embryogenesis Arabidopsis thaliana