Cloned (Comment) | Organism |
---|---|
gene IMPKb | Arabidopsis thaliana |
gene IPMKa, | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a atipk2alpha/atipk2beta double mutant with male gametophyte and embryogenesis defects, the mutant cannot be rescued by either catalytically inactive or substrate specificity-altered variants of AtIPK2beta. The single knock-out mutant of atipk2alpha is indistinguishable from the wild-type. Transmission analysis of atipk2alpha and atipk2beta mutant alleles and Segregation analysis of atipk2alpha and atipk2beta mutants, overview. Complementation analysis of the atipk2alpha/atipk2beta double mutant, development-defective phenotype | Arabidopsis thaliana |
additional information | construction of a atipk2alpha/atipk2beta double mutant with male gametophyte and embryogenesis defects, the mutant cannot be rescued by either catalytically inactive or substrate specificity-altered variants of AtIPK2beta. The single knock-out mutant of atipk2beta is indistinguishable from the wild-type. Transmission analysis of atipk2alpha and atipk2beta mutant alleles and Segregation analysis of atipk2alpha and atipk2beta mutants, overview. Complementation analysis of the atipk2alpha/atipk2beta double mutant, development-defective phenotype | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1-diphosphate pentakisphosphate | Arabidopsis thaliana | - |
ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol hexakisphosphate | Arabidopsis thaliana | - |
ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FLT2 | - |
- |
Arabidopsis thaliana | Q9LY23 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gametophyte | male, isozymes AtIPK2alpha and AtIPK2beta have overlapping expression in male gametophyte | Arabidopsis thaliana | - |
additional information | in vitro and in vivo analysis of pollen germination and pollen tube growth of wild-type and mutant strains, detection of pollen tube guidance in cross-pollination, overview | Arabidopsis thaliana | - |
pollen | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1-diphosphate pentakisphosphate | - |
Arabidopsis thaliana | ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol hexakisphosphate | - |
Arabidopsis thaliana | ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate | - |
? | |
additional information | the consensus PxxxDxKxG motif is responsible for substrate binding and is highly conserved in all known IPK2s | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtIPK2alpha | - |
Arabidopsis thaliana |
AtIpk2beta | - |
Arabidopsis thaliana |
inositol polyphosphate kinase | - |
Arabidopsis thaliana |
Ipk2 | - |
Arabidopsis thaliana |
IPMK | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | expressing either catalytically inactive or substrate specificity-altered variants of AtIPK2beta cannot rescue the male gametophyte and embryogenesis defects of the atipk2alpha/atipk2beta double mutant. The mutation of AtIPK2alpha and AtIPK2beta results in severely reduced transmission of male gametophyte as a result of abnormal pollen development and defective pollen tube guidance. Overexpression or reduction of the expression of AtIPK2alpha and AtIPK2beta reveal their roles in auxiliary shoot branching, abiotic stress responses and root growth. Phenotype, detailed overview | Arabidopsis thaliana |
physiological function | the enzyme is a key component for inositol polyphosphate turnover. The kinase activity of AtIPK2 is required for pollen development, pollen tube guidance and embryogenesis, requirement of inositol polyphosphate signaling in plant sexual reproduction. Isozymes AtIPK2alpha and AtIPK2beta act redundantly during pollen development, pollen tube guidance and embryogenesis | Arabidopsis thaliana |