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Literature summary for 2.7.4.21 extracted from

  • Ghoshal, S.; Tyagi, R.; Zhu, Q.; Chakraborty, A.
    Inositol hexakisphosphate kinase-1 interacts with perilipin1 to modulate lipolysis (2016), Int. J. Biochem. Cell Biol., 78, 149-155.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.21

Application

Application Comment Organism
medicine enzyme IP6K1 is a target in obesity and type-2 diabetes Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
gene IP6K1, recombinant coexpression of GST-tagged wild-type and mutant enzymes with Myc-tagged PLIN1 in HEK-293 cells, recombinant expression of Myc-tagged enzyme Mus musculus

Protein Variants

Protein Variants Comment Organism
S118A/S121A site-directed mutagenesis, no phosphorylation of IP6K1 mutant S118A-S121A in HEK293 cells. The mutant does not efficiently bind Myc-PLIN1 in HEK293 cells Mus musculus
S85A site-directed mutagenesis, the mutation does not influence its binding to Myc-PLIN1 in HEK293 cells Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q6PD10
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-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the phosphorylation of IP6K1 at a PKC/PKA motif modulates its interaction with PLIN1 and lipolysis. GST-IP6K1 phosphorylation in HEK293 cells is enhanced by forskolin-mediated PKA activation. Endogenous IP6K1 phosphorylation in HEK293 cells is enhanced to a greater extent by PKC activation compared to PKA activation as evidenced by PMA and FSK treatments. No phosphorylation of IP6K1 mutant S118A-S121A in HEK293 cells Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
3T3-L1 cell
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 Mus musculus
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adipocyte
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 Mus musculus
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Synonyms

Synonyms Comment Organism
inositol hexakisphosphate kinase-1
-
 Mus musculus
IP6K1
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 Mus musculus

General Information

General Information Comment Organism
malfunction deletion of inositol hexakisphosphate kinase-1 (IP6K1) protects mice from high fat diet induced obesity and insulin resistance in mice. IP6K1-KO mice are lean due to enhanced energy expenditure. IP6K1-KO mice display enhanced basal lipolysis. IP6K1 modulates lipolysis via its interaction with the lipolytic regulator protein perilipin1 (PLIN1) Mus musculus
physiological function inositol hexakisphosphate kinases (IP6Ks) primarily generate the signaling molecule, inositol diphosphate, 5-IP7. Phosphorylation of IP6K1 at a PKC/PKA motif modulates its interaction with PLIN1 and lipolysis. Enzyme IP6K1 is a regulator of PLIN1 mediated lipolysis. The PKA/PKC phosphorylation motif in IP6K1 regulates its interaction with PLIN1 and lipolysis Mus musculus