Cloned (Comment) | Organism |
---|---|
gene GbPMK, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, subcloning and recombinant expression in Escherichia coli strain DH5alpha | Ginkgo biloba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-phosphomevalonate | Ginkgo biloba | - |
ADP + (R)-5-diphosphomevalonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ginkgo biloba | A0A346QPQ2 | collected from Ginkgo Botanical Garden of Yangtze University, Jingzhou, Hubei province, China | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cone | female and male | Ginkgo biloba | - |
fruit | - |
Ginkgo biloba | - |
leaf | - |
Ginkgo biloba | - |
additional information | analysis of expression profile of GbPMK in different tissues. GbPMK exhibits tissue-specific expression, with the highest expression in leaves and the lowest expression in male strobili | Ginkgo biloba | - |
root | - |
Ginkgo biloba | - |
stem | - |
Ginkgo biloba | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-phosphomevalonate | - |
Ginkgo biloba | ADP + (R)-5-diphosphomevalonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55960, sequence calculation | Ginkgo biloba |
Synonyms | Comment | Organism |
---|---|---|
GbPMK | - |
Ginkgo biloba |
PMK | - |
Ginkgo biloba |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ginkgo biloba | sequence calculation | - |
5.74 |
Organism | Comment | Expression |
---|---|---|
Ginkgo biloba | Under methyljasmonate treatment, the expression level of GbPMK significantly decreases in different degrees with prolonged treated time. After 1 d, the expression level of GbPMK is reduced by 0.45fold compared to control | down |
Ginkgo biloba | salicylate generally increases the expression level of GbPMK. The expression first increases, then decreases, and finally reaches the highest level 2 d after the treatment. The expression level is 1.21fold higher than that in the control 2 d after treatments. The expression levels in abscisic acid and ethylene treatments are 3.03 and 1.56fold higher compared to control. After 4 d of treatment, the expression levels decrease to varying degrees | up |
General Information | Comment | Organism |
---|---|---|
evolution | phosphomethoxylate kinase (PMK) is a member of the GHMP kinase superfamily. The GbPMK protein has four conserved domains and one conserved region of the GHMP kinase family. GbPMK possesses a conserved sequence structure and sequence characteristics. PMK phylogenetic and molecular evolution analyses | Ginkgo biloba |
physiological function | phosphomethoxylate kinase (PMK) is one of the core enzyme in the mevalonate pathway, one of the two pathways in plants that can synthase terpenoids. Terpenoids are main active ingredients of Ginkgo biloba | Ginkgo biloba |