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Literature summary for 2.7.4.14 extracted from

  • Kocabas, N.A.; Aksoy, P.; Pelleymounter, L.L.; Moon, I.; Ryu, J.S.; Gilbert, J.A.; Salavaggione, O.E.; Eckloff, B.W.; Wieben, E.D.; Yee, V.; Weinshilboum, R.M.; Ames, M.M.
    Gemcitabine pharmacogenomics: deoxycytidine kinase and cytidylate kinase gene resequencing and functional genomics (2008), Drug Metab. Dispos., 36, 1951-1959.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
transient expression of wild-type and mutant DCK/CMPK enzymes in COS-1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information determination and analysis of 28 polymorphisms in DCK/CMPK enzymes, genotyping with humans of different ethnic origins, overview. Variant allozyme enzyme activities range from 32 to 105% of the wild-type enzyme for DCK and from 78 to 112% of wild-type enzyme for CMPK, with no significant differences in apparent Km values for either enzyme except for a DCK Val24/Ser122 double variant allozyme, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km values of mutant enzymes, overview Homo sapiens
0.0146
-
gemcitabine wild-type DCK Homo sapiens
0.246
-
gemcitabine phosphate wild-type CMPK Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P30085 African-American, Caucasian-American, Han Chinese-American, and Mexican-American subjects
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gemcitabine activity of DCK Homo sapiens ADP + gemcitabine monophosphate i.e. 2',2'-difluorodeoxycytidine 5'-monophosphate ?
ATP + gemcitabine phosphate activity of CMPK Homo sapiens ADP + gemcitabine diphosphate i.e. 2',2'-difluorodeoxycytidine 5'-diphosphate ?

Subunits

Subunits Comment Organism
More DCK and CMPK structural models from crystal structures, overview Homo sapiens

Synonyms

Synonyms Comment Organism
CMPK
-
Homo sapiens
cytidine monophosphate kinase
-
Homo sapiens
dCK
-
Homo sapiens
deoxycytidine kinase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens