Cloned (Comment) | Organism |
---|---|
expression of His-tagged UMP-CMP kinase and UMP-CMP kinase fusion protein with glutathione S-transferase | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CMP | substrate inhibition above 0.2 mM | Homo sapiens | |
UMP | substrate inhibition above 0.2 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
UMP | pH 7.4, 37°C, His-tagged UMP/CMP kinase | Homo sapiens | |
0.022 | - |
CMP | pH 7.4, 37°C | Homo sapiens | |
0.044 | - |
UMP | pH 7.4, 37°C | Homo sapiens | |
0.045 | - |
UMP | pH 7.4, 37°C, His-tagged UMP/CMP kinase | Homo sapiens | |
0.07 | - |
CMP | pH 7.4, 37°C, GST fusion UMP-CMP kinase | Homo sapiens | |
0.14 | - |
UMP | pH 7.4, 37°C, GST fusion UMP-CMP kinase | Homo sapiens | |
0.15 | - |
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate | pH 7.4, 37°C, His-tagged UMP/CMP kinase | Homo sapiens | |
0.2 | - |
CMP | pH 7.4, 37°C | Dictyostelium sp. | |
0.2 | - |
UMP | pH 7.4, 37°C | Dictyostelium sp. | |
0.26 | - |
ara-CMP | pH 7.4, 37°C, His-tagged UMP/CMP kinase | Homo sapiens | |
0.3 | - |
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate | pH 7.4, 37°C, GST fusion UMP-CMP kinase | Homo sapiens | |
0.34 | - |
ara-CMP | pH 7.4, 37°C, GST fusion UMP-CMP kinase | Homo sapiens | |
0.9 | - |
dCMP | pH 7.4, 37°C, His-tagged UMP/CMP kinase | Homo sapiens | |
1.1 | - |
dCMP | pH 7.4, 37°C, GST fusion UMP-CMP kinase | Homo sapiens | |
2 | - |
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate | pH 7.4, 37°C | Dictyostelium sp. | |
2 | - |
cCMP | pH 7.4, 37°C | Dictyostelium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CMP | Homo sapiens | the enzyme catalyses an important step in the phosphorylation of UTP, CTP and dCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies | ADP + CDP | - |
? | |
ATP + dCMP | Homo sapiens | the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies | ADP + dCDP | - |
? | |
ATP + UMP | Homo sapiens | the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies | ADP + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium sp. | - |
- |
- |
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ara-CMP | - |
Homo sapiens | ADP + ara-CDP | - |
? | |
ATP + ara-CMP | - |
Dictyostelium sp. | ADP + ara-CDP | - |
? | |
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate | - |
Homo sapiens | ADP + beta-L-2',3'-dideoxy-3'-thiacytidine diphosphate | - |
? | |
ATP + cCMP | - |
Dictyostelium sp. | ? | - |
? | |
ATP + CMP | - |
Homo sapiens | ADP + CDP | - |
? | |
ATP + CMP | - |
Dictyostelium sp. | ADP + CDP | - |
? | |
ATP + CMP | the enzyme catalyses an important step in the phosphorylation of UTP, CTP and dCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies | Homo sapiens | ADP + CDP | - |
? | |
ATP + dCMP | the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies | Homo sapiens | ADP + dCDP | - |
? | |
ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP | - |
Dictyostelium sp. | ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP | - |
? | |
ATP + UMP | - |
Homo sapiens | ADP + UDP | - |
? | |
ATP + UMP | - |
Dictyostelium sp. | ADP + UDP | - |
? | |
ATP + UMP | the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies | Homo sapiens | ADP + UDP | - |
? | |
additional information | the UMP-CMP kinase has a relaxed enantiospecificity for the nucleoside monophosphate acceptor site, but it is restricted to D-nucleotides at the donor site | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate | pH 7.4, 37°C | Dictyostelium sp. | |
14 | - |
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate | pH 7.4, 37°C, GST fusion UMP/CMP kinase | Homo sapiens | |
22 | - |
dCMP | pH 7.4, 37°C | Dictyostelium sp. | |
30 | - |
dCMP | pH 7.4, 37°C, GST fusion UMP-CMP kinase | Homo sapiens | |
36 | - |
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate | pH 7.4, 37°C, His-tagged UMP-CMP kinase | Homo sapiens | |
45 | - |
ara-CMP | pH 7.4, 37°C, GST fusion UMP-CMP kinase | Homo sapiens | |
72 | - |
CMP | pH 7.4, 37°C, GST fusion UMP-CMP kinase | Homo sapiens | |
73 | - |
dCMP | pH 7.4, 37°C, His-tagged UMP-CMP kinase | Homo sapiens | |
92 | - |
UMP | pH 7.4, 37°C, GST fusion UMP-CMP kinase | Homo sapiens | |
130 | - |
CMP | pH 7.4, 37°C, His-tagged UMP-CMP kinase | Homo sapiens | |
130 | - |
UMP | pH 7.4, 37°C, His-tagged UMP-CMP kinase | Homo sapiens | |
150 | - |
ara-CMP | pH 7.4, 37°C, His-tagged UMP-CMP kinase | Homo sapiens | |
217 | - |
UMP | pH 7.4, 37°C | Dictyostelium sp. | |
410 | - |
CMP | pH 7.4, 37°C | Dictyostelium sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
CMP | pH 7.4, 37°C, GST fusion UMP/CMP kinase | Homo sapiens | |
0.5 | - |
CMP | pH 7.4, 37°C, His-tagged UMP/CMP kinase | Homo sapiens | |
0.5 | - |
UMP | pH 7.4, 37°C, GST fusion UMP/CMP kinase | Homo sapiens | |
1.5 | - |
UMP | pH 7.4, 37°C, His-tagged UMP/CMP kinase | Homo sapiens |