Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. The 228-aa and the 196-11 form are expressed as His-tagged fusion protein. The 196-aa UMP/CMP kinase is the actual form of the enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CTP | inhibits reaction with ATP and UMP, CMP or dCMP | Homo sapiens | |
dCTP | inhibits reaction with ATP and UMP, CMP or dCMP | Homo sapiens | |
TTP | inhibits reaction with ATP and UMP, CMP or dCMP | Homo sapiens | |
UTP | inhibits reaction with ATP and UMP, CMP or dCMP | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
CMP | 37°C, 196-aa and 228-aa enzyme form, in presence of 8 mM MgATP2- | Homo sapiens | |
0.023 | - |
dCMP | 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- | Homo sapiens | |
0.067 | - |
UMP | 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- | Homo sapiens | |
0.069 | - |
UMP | 37°C, 228-aa enzyme form, in presence of 8 mM MgATP2- | Homo sapiens | |
0.204 | - |
ATP | 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- | Homo sapiens | |
0.211 | - |
CMP | 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- | Homo sapiens | |
0.228 | - |
beta-L-(-)-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP | 37°C | Homo sapiens | |
0.25 | - |
L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP | 37°C, His-tagged UMP/CMP kinase | Homo sapiens | |
0.272 | - |
beta-D-2',3'-dideoxy-CMP | 37°C | Homo sapiens | |
0.354 | - |
dCMP | 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- | Homo sapiens | |
0.494 | - |
L-(-)-2',3'-dideoxy-3'-thia-CMP | 37°C | Homo sapiens | |
0.513 | - |
dCMP | 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- | Homo sapiens | |
0.581 | - |
gemcitabine monophosphate | 37°C | Homo sapiens | |
0.697 | - |
beta-L-2',3'-dideoxy-CMP | 37°C | Homo sapiens | |
0.917 | - |
ara-CMP | 37°C | Homo sapiens | |
1.037 | - |
beta-D-2',3'-dideoxy-CMP | 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CMP | Homo sapiens | the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis | ADP + CDP | - |
? | |
ATP + dCMP | Homo sapiens | the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis | ADP + dCDP | - |
? | |
ATP + UMP | Homo sapiens | the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis | ADP + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa-S3 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ara-CMP | - |
Homo sapiens | ADP + ara-CDP | - |
? | |
ATP + beta-D-2',3'-dideoxy-CMP + H2O | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + beta-L-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + beta-L-2',3'-dideoxy-CMP + H2O | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + beta-L-dioxolane-cytidine | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + CMP | - |
Homo sapiens | ADP + CDP | - |
? | |
ATP + CMP | the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis | Homo sapiens | ADP + CDP | - |
? | |
ATP + dCMP | - |
Homo sapiens | ADP + dCDP | - |
? | |
ATP + dCMP | the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis | Homo sapiens | ADP + dCDP | - |
? | |
ATP + gemcitabine monophosphate | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP | - |
Homo sapiens | ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP | - |
? | |
ATP + L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + UMP | - |
Homo sapiens | ADP + UDP | - |
? | |
ATP + UMP | the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis | Homo sapiens | ADP + UDP | - |
? | |
CTP + CMP | - |
Homo sapiens | CDP + CDP | - |
? | |
dATP + CMP | - |
Homo sapiens | dADP + CDP | - |
? | |
dATP + UMP | - |
Homo sapiens | dADP + ADP | - |
? | |
dCTP + dCMP | - |
Homo sapiens | dCDP + dCDP | - |
? | |
dCTP + UMP | - |
Homo sapiens | dGDP + UDP | - |
? | |
dGTP + CMP | - |
Homo sapiens | dGDP + CDP | - |
? | |
dGTP + UMP | - |
Homo sapiens | dGDP + UDP | - |
? | |
GTP + CMP | - |
Homo sapiens | GDP + CDP | - |
? | |
GTP + dCMP | - |
Homo sapiens | GDP + dCDP | - |
? | |
GTP + UMP | - |
Homo sapiens | GDP + UDP | - |
? | |
TTP + CMP | - |
Homo sapiens | TDP + CDP | - |
? | |
TTP + dCMP | - |
Homo sapiens | TDP + dCMP | - |
? | |
TTP + UMP | - |
Homo sapiens | TDP + UDP | - |
? |