Protein Variants | Comment | Organism |
---|---|---|
additional information | two types of chimeric enzymes have been constructed by genetic engineering of chicken cytosolic adenylate kinase and porcine brain UMP/CMP kinase. One designated as UAU carries an AMP-binding domain of AK in the remaining body of UMP/CMP kinase, and the other, designated as AUA, carries a UMP/CMP-binding domain in the remaining body of adenylate kinase. UAU is 4fold more active with AMP, 40fold less active with UMP, and 4fold less active with CMP than the parental UMP/CMP kinase, although AUA has considerably lowered reactivity for both AMP and UMP. AUA has a Tm-value 11°C lower than adenylate kinase, whereas UAU has a Tm-value similar to that of UMP/CMP kinase. Expression in Escherichia coli JM109 | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CMP | - |
Sus scrofa | ADP + CDP | - |
? | |
ATP + UMP | - |
Sus scrofa | ADP + UDP | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
midpoint denaturation temperature of the AUA chimeric enzyme | Sus scrofa |
51 | - |
midpoint denaturation temperature of UMP/CMP kinase | Sus scrofa |
52 | - |
midpoint denaturation temperature of the UAU chimeric enzyme | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
UMP | pH 7.0, 25°C, AUA chimeric enzyme | Sus scrofa | |
13.7 | - |
UMP | pH 7.0, 25°C, UAU chimeric enzyme | Sus scrofa | |
174 | - |
UMP | pH 7.0, 25°C, UMP-CMP kinase | Sus scrofa |