Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.4.14 extracted from

  • Van Rompay, A.R.; Johansson, M.; Karlsson, A.
    Phosphorylation of deoxycytidine analog monophosphates by UMP-CMP kinase: molecular characterization of the human enzyme (1999), Mol. Pharmacol., 56, 562-569.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. The 228-aa and the 196-11 form are expressed as His-tagged fusion protein. The 196-aa UMP/CMP kinase is the actual form of the enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
2',2'-difluorodeoxycytidine pH 8.0, 37°C Homo sapiens
0.5
-
CMP pH 8.0, 37°C Homo sapiens
1.4
-
araCMP pH 8.0, 37°C Homo sapiens
1.6
-
UMP pH 8.0, 37°C Homo sapiens
5.9
-
dUMP pH 8.0, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
nucleus
-
Homo sapiens 5634
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P30085
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-beta-D-arabinofuranosylcytosine
-
Homo sapiens ADP + ?
-
?
ATP + 2',2'-difluorodeoxycytidine
-
Homo sapiens ADP + ?
-
?
ATP + AMP
-
Homo sapiens ADP + ADP
-
r
ATP + ara-CMP
-
Homo sapiens ADP + ara-CDP
-
?
ATP + CMP
-
Homo sapiens ADP + CDP
-
?
ATP + dAMP
-
Homo sapiens ADP + dADP
-
?
ATP + dCMP
-
Homo sapiens ADP + dCDP
-
?
ATP + dUMP
-
Homo sapiens ADP + dUDP
-
?
ATP + UMP
-
Homo sapiens ADP + UDP
-
?