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Literature summary for 2.7.4.14 extracted from

  • Zhou, L.; Lacroute, F.; Thornburg, R.
    Cloning, expression in Escherichia coli, and characterization of Arabidopsis thaliana UMP/CMP kinase (1998), Plant Physiol., 117, 245-254.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cDNA is subcloned into pGEX-4T-3 and expressed as a glutathione S-transferase fusion protein in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
P1,P5-di(adenosine-5')pentaphosphate
-
Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
ATP pH 6.5, when UMP is the other substrate Arabidopsis thaliana
0.153
-
UMP pH 6.5 Arabidopsis thaliana
0.266
-
CMP pH 6.5 Arabidopsis thaliana
0.292
-
ATP pH 6.5, reaction with CMP Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22448
-
x * 22448, mass spectroscopy, calculation from nucleotide sequence Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O04905
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana

Reaction

Reaction Comment Organism Reaction ID
ATP + UMP = ADP + UDP random bi-bi mechanism Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + CMP
-
Arabidopsis thaliana ADP + CDP
-
?
ATP + UMP
-
Arabidopsis thaliana ADP + UDP
-
?

Subunits

Subunits Comment Organism
? x * 22448, mass spectroscopy, calculation from nucleotide sequence Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
10 min, 50% loss of activity Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.64
-
UMP pH 6.5 Arabidopsis thaliana
8.57
-
CMP pH 6.5 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0012
-
P1,P5-di(adenosine-5') pentaphosphate versus ATP Arabidopsis thaliana
0.00653
-
P1,P5-di(adenosine-5') pentaphosphate versus UMP Arabidopsis thaliana