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Literature summary for 2.7.4.14 extracted from

  • Kohno, H.; Kumagai, H.; Tochikura, T.
    Purification and properties of pyrimidine nucleoside monophosphate kinase from baker‘s yeast (1983), Agric. Biol. Chem., 47, 19-24.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activates Saccharomyces cerevisiae
dithiothreitol 1 mM, activates Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
CMP competitive inhibition of UMP phosphorylation Saccharomyces cerevisiae
dCMP competitive inhibition of UMP phosphorylation Saccharomyces cerevisiae
HgCl2 0.1 mM, 88% inhibition Saccharomyces cerevisiae
NEM 1.0 mM; 41% inhibition Saccharomyces cerevisiae
PCMB 0.1 mM, 69% loss of activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
UMP pH 7.5, 25°C Saccharomyces cerevisiae
0.071
-
CMP pH 7.5, 25°C Saccharomyces cerevisiae
0.54
-
dCMP pH 7.5, 25°C Saccharomyces cerevisiae
8.5
-
dUMP pH 7.5, 25°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required, Mn2+ can substitute for Mg2+ Saccharomyces cerevisiae
Mg2+ divalent cation required, Mg2+ is most effective Saccharomyces cerevisiae
Mn2+ divalent cation required, Mn2+ can substitute for Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.1
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + CMP
-
Saccharomyces cerevisiae ADP + CDP
-
?
ATP + dCMP
-
Saccharomyces cerevisiae ADP + dCDP
-
?
ATP + dUMP
-
Saccharomyces cerevisiae ADP + dUDP
-
?
ATP + UMP
-
Saccharomyces cerevisiae ADP + UDP
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
at pH 7 the enzyme is most stable when kept at 4°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
reaction with UMP and ATP Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
4 9 pH 4.0: about 40% of maximal activity, pH 9.0: about 65% of maximal activity, reaction with UMP and ATP Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
at pH 7.0, the enzyme is most stable when kept at 4°C Saccharomyces cerevisiae