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Literature summary for 2.7.4.14 extracted from

  • Seagrave, J.; Reyes, P.
    Pyrimidine nucleoside monophosphate kinase from rat bone marrow cells: chromatographic, electrophoretic, and sedimentation behavior of active and inactive enzyme forms (1986), Arch. Biochem. Biophys., 247, 76-83.
    View publication on PubMed

General Stability

General Stability Organism
dialysis against 20 mM phosphate, 1 mM MgCl2, 20% ethylene glycol, pH 8.0, 90% loss of activity Rattus norvegicus
freeze-thawing inactivates Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
DTNB 0.009 mM, 50% inhibition Rattus norvegicus
iodoacetate 0.05 mM, 50% inhibition Rattus norvegicus
NEM 0.035 mM, 50% inhibition Rattus norvegicus
p-hydroxymercuribenzoate 0.02 mM, 50% inhibition Rattus norvegicus
p-Hydroxymercuriphenyl sulfonate 0.02 mM, 50% inhibition Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22500
-
sucrose density gradient centrifugation Rattus norvegicus
26000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
bone marrow
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-80°C, up to 12 months Rattus norvegicus
4°C, considerable loss of activity within 24 h, DTT stabilizes, more stable in 20 mM phosphate buffer, pH 8 than in Tris-HCl buffer Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
24 h, in absence of dithiothreitol the purified enzyme shows considerable loss of activity Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus isoelectric focusing 4.8 4.7