Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.4.14 extracted from

  • Teng, Y.S.; Chen, S.H.; Scott, C.R.
    Human erythrocyte pyrimidine nucleoside monophosphate kinase. Partial purification and properties of two allelic gene products (1976), J. Biol. Chem., 251, 4179-4183.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 0.25 mM, 96% inhibition of enzyme form UMPK1 and 92% inhibition of enzyme form UMPK2 Homo sapiens
HgCl2 0.25 mM, complete inhibition of enzyme form UMPK1 and UMPK2 Homo sapiens
lead nitrate 0.25 mM, 52% inhibition of enzyme form UMPK1 and 40% inhibition of enzyme form UMPK2 Homo sapiens
ZnCl2 0.25 mM, 55% inhibition of enzyme form UMPK1 and 30% inhibition of enzyme form UMPK2 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
CMP 37°C enzyme form UMPK 1 Homo sapiens
0.028
-
CMP 37°C, enzyme form UMPK2 Homo sapiens
0.053
-
UMP 37°C, enzyme form UMPK1 Homo sapiens
0.064
-
UMP 37°C, enzyme form UMPK2 Homo sapiens
0.25
-
ATP 37°C, enzyme form UMPK2 Homo sapiens
0.36
-
ATP 37°C, enzyme form UMPK1 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, two allelic gene products UMPK1 and UMPK2 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AMP
-
Homo sapiens ADP + ADP
-
r
ATP + CMP
-
Homo sapiens ADP + CDP
-
?
ATP + dUMP
-
Homo sapiens ADP + dUDP
-
?
ATP + UMP
-
Homo sapiens ADP + UDP
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
30 min, stable Homo sapiens
40
-
30 min, enzyme form UMPK2 loses 70% of maximal activity, enzyme form UMPK1 loses 30% of its activity Homo sapiens
45
-
30 min, enzyme form UMPK2 loses more than 80% of maximal activity, enzyme form UMPK1 loses more than 60% of its activity Homo sapiens
50
-
30 min, enzyme forms UMPK2 and UMPK1 lose 90% of its activity Homo sapiens