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Literature summary for 2.7.4.14 extracted from

  • Scott, E.M.; Wright, R.C.
    Kinetics and equilibria of pyrimidine nucleoside monophosphate kinase from human erythrocytes (1979), Biochim. Biophys. Acta, 571, 45-54.
    View publication on PubMed

General Stability

General Stability Organism
more stable in histidine than in phosphate buffer Homo sapiens
the purified enzyme is notably unstable Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration is 2-3 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + UMP = ADP + UDP formation of a ternary complex, addition of substrates is random Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
337
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, concentrated enzyme solution in 30% glycerol, less than 5% loss of activity per month Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AMP
-
Homo sapiens ADP + ADP
-
r
ATP + CMP
-
Homo sapiens ADP + CDP
-
r
ATP + UMP
-
Homo sapiens ADP + UDP
-
r
dATP + UMP
-
Homo sapiens dADP + ADP
-
r
additional information formation of a ternary complex, addition of substrates is random Homo sapiens ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
in absence of glycerol, the half-life of a preparation with a specific activity of 80 is about 10 min, inactivation is partially reversed by addition of 2-mercaptoethanol Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
more stable in histidine buffer than in phosphate buffer Homo sapiens
6.5
-
more stable than at higher pH Homo sapiens