Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | activity is strictly dependent upon sulfhydryl reducing agents. Reduced thioredoxin is by far the most effective | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CMP | above 0.13 mM, substrate inhibition | Rattus norvegicus | |
F- | complete inhibition at 25 mM | Rattus norvegicus | |
additional information | no substrate inhibition with 3 mM dCMP and 1 mM UMP | Rattus norvegicus | |
NaClO | 250 mM | Rattus norvegicus | |
NaSCN | 250 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0053 | - |
CMP | reaction with ATP, enzyme from Novikoff ascites hepatoma | Rattus norvegicus | |
0.027 | - |
dCMP | reaction with dATP, liver enzyme | Rattus norvegicus | |
0.03 | - |
CMP | reaction with ATP, liver enzyme | Rattus norvegicus | |
0.04 | - |
UMP | reaction with ATP, liver enzyme | Rattus norvegicus | |
0.043 | - |
UMP | reaction with ATP, enzyme from Novikoff ascites hepatoma | Rattus norvegicus | |
0.053 | - |
UMP | reaction with dATP, liver enzyme | Rattus norvegicus | |
0.074 | - |
dATP | reaction with CMP, liver enzyme | Rattus norvegicus | |
0.134 | - |
ATP | reaction with dCMP, enzyme from Novikoff ascites hepatoma | Rattus norvegicus | |
0.32 | - |
ATP | reaction with CMP, liver enzyme | Rattus norvegicus | |
0.42 | - |
dATP | reaction with UMP, liver enzyme | Rattus norvegicus | |
0.58 | - |
ATP | reaction with UMP, liver enzyme | Rattus norvegicus | |
0.61 | - |
dATP | reaction with dCMP, liver enzyme | Rattus norvegicus | |
0.68 | - |
ATP | reaction with dCMP, liver enzyme | Rattus norvegicus | |
0.715 | - |
dCMP | reaction with ATP, enzyme from Novikoff ascites hepatoma | Rattus norvegicus | |
0.82 | - |
dCTP | reaction with CMP, liver enzyme | Rattus norvegicus | |
0.98 | - |
CMP | reaction with dCTP, liver enzyme | Rattus norvegicus | |
1.1 | - |
dCMP | reaction with dATP, liver enzyme | Rattus norvegicus | |
2.77 | - |
dCMP | reaction with ATP, liver enzyme | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) | Rattus norvegicus | |
Mg2+ | required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) | Rattus norvegicus | |
Mn2+ | required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) | Rattus norvegicus | |
Na2SO4 | 250 mM, stimulates | Rattus norvegicus | |
NaCl | 250 mM, stimulates | Rattus norvegicus | |
Ni2+ | required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Novikoff ascites hepatoma cell | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.2 | - |
reaction with CMP and ATP | Rattus norvegicus |
4.98 | - |
reaction with dCMP and ATP | Rattus norvegicus |
8.76 | - |
reaction with UMP and dCMP | Rattus norvegicus |
30 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, in 25 mM Tris-acetate buffer, pH 7.5, 50 mM 2-mercaptoethanol, 50% glycerol, stable for at least 2 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + CMP | - |
Rattus norvegicus | ADP + CDP | - |
? | |
ATP + dCMP | - |
Rattus norvegicus | ADP + dCDP | - |
? | |
ATP + UMP | - |
Rattus norvegicus | ADP + UDP | - |
? | |
dATP + CMP | - |
Rattus norvegicus | dADP + CDP | - |
? | |
dATP + dCMP | - |
Rattus norvegicus | dADP + dCDP | - |
? | |
dATP + UMP | - |
Rattus norvegicus | dADP + ADP | - |
? | |
dCTP + CMP | - |
Rattus norvegicus | dCDP + CDP | - |
? | |
ITP + UMP | - |
Rattus norvegicus | IDP + UDP | - |
? |