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Literature summary for 2.7.3.4 extracted from
- Phosphagen kinase of the giant tubeworm Riftia pachyptila. Cloning and expression of cytoplasmic and mitochondrial isoforms of taurocyamine kinase (2005), Int. J. Biol. Macromol., 37, 54-60.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli TB1 | Riftia pachyptila |
General Stability
| General Stability | Organism |
|---|---|
| structurally very unstable, gradual loss of enzyme activity even when it is stored on ice for several hours | Riftia pachyptila |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.881 | - |
Taurocyamine | - |
Arenicola brasiliensis |  |
| 2.12 | - |
Taurocyamine | - |
Riftia pachyptila | |
| 4.01 | - |
Taurocyamine | - |
Arenicola brasiliensis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Riftia pachyptila | 5737 | - |
| cytoplasm | - |
Arenicola brasiliensis | 5737 | - |
| mitochondrion | - |
Riftia pachyptila | 5739 | - |
| mitochondrion | - |
Arenicola brasiliensis | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40830 | - |
calculated from amino acid sequence | Riftia pachyptila |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + taurocyamine | Riftia pachyptila | key role in the interconnection between energy production and utilization in animals | ADP + N-phosphotaurocyamine | - |
? | |
| ATP + taurocyamine | Arenicola brasiliensis | key role in the interconnection between energy production and utilization in animals | ADP + N-phosphotaurocyamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arenicola brasiliensis | - |
- |
- |
| Riftia pachyptila | Q4AEC7 | - |
- |
| Riftia pachyptila | Q4AEC8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein is purified by affinity chromatography using amylose resin | Riftia pachyptila |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| plume | a small part of the plume | Riftia pachyptila | - |
| vestimentum | - |
Riftia pachyptila | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.298 | - |
arginine | Riftia pachyptila |
| 0.855 | - |
lombricine | Riftia pachyptila |
| 3.25 | - |
lombricine | Riftia pachyptila |
| 3.65 | - |
glycocyamine | Riftia pachyptila |
| 4.04 | - |
taurocyamine | Riftia pachyptila |
| 10.4 | - |
taurocyamine | Riftia pachyptila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + arginine | 2.9% of taurocyamine activity | Riftia pachyptila | ADP + ? | - |
? | |
| ATP + glycocyamine | no activity | Riftia pachyptila | ADP + N-phosphoglycocyamine | - |
? | |
| ATP + glycocyamine | 35% of taurocyamine activity | Riftia pachyptila | ADP + N-phosphoglycocyamine | - |
? | |
| ATP + lombricine | 21% of taurocyamine activity | Riftia pachyptila | ADP + N-phospholombricine | - |
? | |
| ATP + lombricine | 31% of taurocyamine activity | Riftia pachyptila | ADP + N-phospholombricine | - |
? | |
| ATP + taurocyamine | - |
Riftia pachyptila | ADP + N-phosphotaurocyamine | - |
? | |
| ATP + taurocyamine | - |
Arenicola brasiliensis | ADP + N-phosphotaurocyamine | - |
? | |
| ATP + taurocyamine | key role in the interconnection between energy production and utilization in animals | Riftia pachyptila | ADP + N-phosphotaurocyamine | - |
? | |
| ATP + taurocyamine | key role in the interconnection between energy production and utilization in animals | Arenicola brasiliensis | ADP + N-phosphotaurocyamine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MiTK | - |
Arenicola brasiliensis |
| phosphagen kinase | - |
Riftia pachyptila |
| taurocyamine kinase | - |
Riftia pachyptila |
| taurocyamine kinase | - |
Arenicola brasiliensis |
| TK | - |
Arenicola brasiliensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.43 | - |
Taurocyamine | - |
Arenicola brasiliensis | |
| 12.5 | - |
Taurocyamine | - |
Riftia pachyptila | |
| 14.3 | - |
Taurocyamine | - |
Arenicola brasiliensis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Riftia pachyptila | calculated | - |
7.98 |
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