Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Codon Plus cells | Locusta migratoria manilensis |
Protein Variants | Comment | Organism |
---|---|---|
I121D | the mutation leads to pronounced loss of activity and structural stability. The mutation also leads to serious aggregation during heat-and guanidine hydrochloride-induced denaturation and refolding from the guanidine hydrochloride-denatured state | Locusta migratoria manilensis |
I121G | the mutation leads to pronounced loss of activity and structural stability. The mutation also leads to serious aggregation during heat-and guanidine hydrochloride-induced denaturation and refolding from the guanidine hydrochloride-denatured state | Locusta migratoria manilensis |
I121K | the mutation leads to pronounced loss of activity and structural stability. The mutation also leads to serious aggregation during heat-and guanidine hydrochloride-induced denaturation and refolding from the guanidine hydrochloride-denatured state | Locusta migratoria manilensis |
I121L | the almost has no effect on AK activity and structural stability | Locusta migratoria manilensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.94 | - |
L-Arg | native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
0.951 | - |
L-Arg | recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
0.98 | - |
L-Arg | mutant enzyme I121L, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
1.27 | - |
ATP | recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
1.29 | - |
ATP | native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
1.36 | - |
ATP | mutant enzyme I121L, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
1.74 | - |
L-Arg | mutant enzyme I121G, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
2.38 | - |
ATP | mutant enzyme I121G, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
2.5 | 3 | L-Arg | mutant enzyme I121K, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
3.04 | - |
L-Arg | mutant enzyme I121D, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
3.56 | - |
ATP | mutant enzyme I121K, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
4.06 | - |
ATP | mutant enzyme I121D, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, calculated from amino acid sequence | Locusta migratoria manilensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Locusta migratoria manilensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Locusta migratoria manilensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Arg | - |
Locusta migratoria manilensis | ADP + Nomega-phospho-L-Arg | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, calculated from amino acid sequence | Locusta migratoria manilensis |
Synonyms | Comment | Organism |
---|---|---|
ATP: L-arginine phosphotransferase | - |
Locusta migratoria manilensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 65 | the wild type enzyme can retain its activity well at temperatures lower than 48°C, and then its activity shows a steep decrease from 45 to 60°C and completely loses its activity at temperatures above 65°C. The melting temperature is at about 56°C | Locusta migratoria manilensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60.36 | - |
L-Arg | mutant enzyme I121D, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
70.93 | - |
L-Arg | mutant enzyme I121K, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
109.8 | - |
L-Arg | mutant enzyme I121G, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
151.2 | - |
L-Arg | mutant enzyme I121L, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
159.4 | - |
L-Arg | recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis | |
163 | - |
L-Arg | native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C | Locusta migratoria manilensis |