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Literature summary for 2.7.3.2 extracted from

  • Zhang, J.W.; Guo, Q.; Zhao, T.J.; Liu, T.T.; Wang, X.C.
    Two fused proteins combining Stichopus japonicus arginine kinase and rabbit muscle creatine kinase (2006), Biochemistry (Moscow), 71, 983-988.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information fusion proteins of Stichopus japonicus arginine kinase and rabbit muscle creatine kinase in direction arginine kinase-creatine kinase, AK-CK and creatine kinase-arginine kinase, CK-AK. In both fusion proteins, both of the enzymes show about 50% decrease in activity and about 2fold Km values. Fused proteins have similar secondary structure, tertiary structure, molecular size, and thermodynamic stability Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
ATP wild-type Oryctolagus cuniculus
0.73
-
ATP fusion protein CK-AK Oryctolagus cuniculus
0.86
-
ATP fusion protein AK-CK Oryctolagus cuniculus
6.4
-
Creatine wild-type Oryctolagus cuniculus
13
-
Creatine fusion protein CK-AK Oryctolagus cuniculus
14
-
Creatine fusion protein AK-CK Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + creatine
-
Oryctolagus cuniculus ADP + creatine phosphate
-
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