Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, mostly in insoluble form, after optimization of the conditions the enzyme does not aggregate and shows multi-kinetic phases | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | prevents aggregation of the enzyme during recombinant expression in Escherichia coli | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
insoluble recombinant enzyme from Escherichia coli by 6 M urea, unfolding shows biphasic kinetic courses, aggregation during refolding follows first-order kinetics, refolding intermediates are stabilized by NaCl, refolded enzyme shows high specific activity | Homo sapiens |