Any feedback?
Literature summary for 2.7.3.2 extracted from
- Studies on the stability of creatine kinase isozymes (2003), Biochem. Cell Biol., 81, 9-16.
General Stability
| General Stability | Organism |
|---|---|
| isozyme CK-MM is more stable against unfolding by urea up to 3 M, while isozymes CK-BB and CK-MB unfold at lower urea concentrations of 2 M, at 25°C and pH 8.0 | Oryctolagus cuniculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | isozymes CK-MM, CK-BB, and CK-MB | Oryctolagus cuniculus | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | Oryctolagus cuniculus | - |
ADP + phosphocreatine | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
isozymes CK-MM, CK-BB, and CK-MB | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| freeze drying leads to oxidation of isozyme CK-BB | Oryctolagus cuniculus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| freeze drying leads to rearrangement of isozyme CK-MB after dissociation of the subunits | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Oryctolagus cuniculus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| freeze drying leads to oxidation of isozyme CK-BB and rearrangement of isozyme CK-MB | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + creatine | - |
Oryctolagus cuniculus | ADP + phosphocreatine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Oryctolagus cuniculus |
| More | the isozymes form monomers upon freeze-drying due to loss of subunit interactions | Oryctolagus cuniculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
temperature stability profile of the isozymes CK-MM, CK-BB, and CK-MB, overview | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Oryctolagus cuniculus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
