Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.3.2 extracted from

  • Schlegel, J.; Zurbriggen, B.; Wegmann, G.; Wyss, M.; Eppenberger, H.M.; Wallimann, T.
    Native mitochondrial creatine kinase forms octameric structures. I. Isolation of two interconvertible mitochondrial creatine kinase forms, dimeric and octameric mitochondrial creatine kinase: characterization, localization, and structure-function relationships (1988), J. Biol. Chem., 263, 16942-16953.
    View publication on PubMed
Search for more literature for 2.7.3.2

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Gallus gallus 5737
-
mitochondrion accumulated in contact sites between inner and outer mitochondrial membrane Gallus gallus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, SDS-PAGE Gallus gallus
42000
-
 8 * 42000, SDS-PAGE, octameric structure dissociates during storage at -20°C, pH above 8.5, protein concentration below 0.3 mg/ml to dimeric form Gallus gallus
360000
-
 isozyme Mia-CK, octameric form, gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Gallus gallus

Storage Stability

Storage Stability Organism
4°C, octameric enzyme, protein concentration above 1 mg/ml, 1 mM 2-mercaptoethanol, 0.2 mM EDTA, 0.26 M NaCl, 25 mM sodium phosphate buffer, pH 7.0 Gallus gallus
liquid N2 preserves octameric stucture, dissociation to dimer at higher temperatures Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + creatine
-
 Gallus gallus ADP + creatine phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Gallus gallus
octamer 8 * 42000, SDS-PAGE, octameric structure dissociates during storage at -20°C, pH above 8.5, protein concentration below 0.3 mg/ml to dimeric form Gallus gallus