Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Campylobacter jejuni |
Protein Variants | Comment | Organism |
---|---|---|
H737N | inactive | Campylobacter jejuni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.64 | - |
L-glutamine | at pH 8.0 and 25°C | Campylobacter jejuni | |
1.5 | - |
gamma-L-glutamyl hydroxamate | at pH 8.0 and 25°C | Campylobacter jejuni | |
10.5 | - |
D-glutamine | at pH 8.0 and 25°C | Campylobacter jejuni | |
17.2 | - |
gamma-L-glutamyl hydrazide | at pH 8.0 and 25°C | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamine + H2O | Campylobacter jejuni | - |
AMP + phosphate + N5-phospho-L-glutamine | - |
r | |
ATP + L-glutamine + H2O | Campylobacter jejuni ATCC 700819 | - |
AMP + phosphate + N5-phospho-L-glutamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | Q0P8J6 | - |
- |
Campylobacter jejuni ATCC 700819 | Q0P8J6 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Sephadex gel filtration | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-L-aspartyl hydroxamate + H2O | - |
Campylobacter jejuni | AMP + phosphate + N5-phospho-beta-L-aspartyl hydroxamate | - |
? | |
ATP + beta-L-aspartyl hydroxamate + H2O | - |
Campylobacter jejuni ATCC 700819 | AMP + phosphate + N5-phospho-beta-L-aspartyl hydroxamate | - |
? | |
ATP + D-glutamine + H2O | - |
Campylobacter jejuni | AMP + phosphate + N5-phospho-D-glutamine | - |
? | |
ATP + D-glutamine + H2O | - |
Campylobacter jejuni ATCC 700819 | AMP + phosphate + N5-phospho-D-glutamine | - |
? | |
ATP + gamma-L-glutamyl hydrazide + H2O | - |
Campylobacter jejuni | AMP + phosphate + N5-phospho-gamma-L-glutamyl hydrazide | - |
? | |
ATP + gamma-L-glutamyl hydroxamate + H2O | second best substrate | Campylobacter jejuni | AMP + phosphate + N5-phospho-gamma-L-glutamyl hydroxamate | - |
? | |
ATP + L-glutamine + H2O | - |
Campylobacter jejuni | AMP + phosphate + N5-phospho-L-glutamine | - |
r | |
ATP + L-glutamine + H2O | best substrate | Campylobacter jejuni | AMP + phosphate + N5-phospho-L-glutamine | - |
r | |
ATP + L-glutamine + H2O | - |
Campylobacter jejuni ATCC 700819 | AMP + phosphate + N5-phospho-L-glutamine | - |
r | |
ATP + L-glutamine + H2O | best substrate | Campylobacter jejuni ATCC 700819 | AMP + phosphate + N5-phospho-L-glutamine | - |
r | |
additional information | no activity with L-glutamate and L-asparagine | Campylobacter jejuni | ? | - |
- |
|
additional information | no activity with L-glutamate and L-asparagine | Campylobacter jejuni ATCC 700819 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
Cj1418 | - |
Campylobacter jejuni |
L-glutamine kinase | - |
Campylobacter jejuni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
gamma-L-glutamyl hydrazide | at pH 8.0 and 25°C | Campylobacter jejuni | |
1.5 | - |
D-glutamine | at pH 8.0 and 25°C | Campylobacter jejuni | |
2.1 | - |
gamma-L-glutamyl hydroxamate | at pH 8.0 and 25°C | Campylobacter jejuni | |
2.5 | - |
L-glutamine | at pH 8.0 and 25°C | Campylobacter jejuni |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
beta-L-aspartyl hydroxamate | at pH 8.0 and 25°C | Campylobacter jejuni | |
0.024 | - |
gamma-L-glutamyl hydrazide | at pH 8.0 and 25°C | Campylobacter jejuni | |
0.14 | - |
D-glutamine | at pH 8.0 and 25°C | Campylobacter jejuni | |
1.4 | - |
gamma-L-glutamyl hydroxamate | at pH 8.0 and 25°C | Campylobacter jejuni | |
3.9 | - |
L-glutamine | at pH 8.0 and 25°C | Campylobacter jejuni |