Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as maltose-binding protein fusion proteins | Namalycastis sp. |
General Stability | Organism |
---|---|
the recombinant GK alpha enzyme is quite unstable, storage on ice for 4 h caused 10% loss of activity, in contrast GK beta enzyme is stable, the instability of GK alpha is most likely due to its expression as a maltose-binding protein fusion protein | Namalycastis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.89 | - |
ATP | native GK | Namalycastis sp. | |
1.02 | - |
ATP | recombinant GK beta | Namalycastis sp. | |
3.38 | - |
glycocyamine | recombinant GK beta | Namalycastis sp. | |
4.7 | - |
glycocyamine | native GK | Namalycastis sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Namalycastis sp. | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | essential for activation | Namalycastis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Namalycastis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + guanidinoacetate | Namalycastis sp. | - |
ADP + phosphoguanidinoacetate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Namalycastis sp. | Q6AW42 | beta chain | - |
Namalycastis sp. | Q6AW43 | alpha chain | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, gel filtration on Sephacryl S-200n, and DEAE-5PW ion exchange chromatography | Namalycastis sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
body wall muscle | - |
Namalycastis sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | 0.5% of the activity with glycocyamine | Namalycastis sp. | ADP + phosphocreatine | - |
r | |
ATP + glycocyamine | - |
Namalycastis sp. | ADP + phosphoglycocyamine | - |
r | |
ATP + guanidinoacetate | - |
Namalycastis sp. | ADP + phosphoguanidinoacetate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | alphabeta, 2 * ca. 40000, SDS-PAGE | Namalycastis sp. |
Synonyms | Comment | Organism |
---|---|---|
GK | - |
Namalycastis sp. |
glycocyamine kinase | - |
Namalycastis sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
glycocyamine | recombinant GK beta | Namalycastis sp. | |
25.3 | - |
glycocyamine | native GK | Namalycastis sp. |