Application | Comment | Organism |
---|---|---|
additional information | ASK1 and ASK2 share a high degree of identity with each other, there is one amino acid difference in the Ask2 enzymes of Oh545o2 and Oh51Ao2 | Zea mays |
Cloned (Comment) | Organism |
---|---|
the monofunctional AK, Ask1 and Ask2 coding sequences are cloned using RNA from the B73 inbred, the two maize genes share a high degree of identity with each other and similar genes of other species, Ask2-Oh545o2 and Ask2-Oh51Ao2 sequences are expressed in Saccharomyces cerevisiae hom3/ura3 mutant sigma a3hu, there is one amino acid difference between the Ask2 alleles, Ask2-Oh545o2 and Ask2-Oh51Ao2 have similar lysine-sensitivity properties but differ in basal activity, the Ask2 gene is tightly linked to the QTL on chromosome 2 that is associated with a high endosperm lysine content. There is one amino acid difference in the Ask2 enzymes of Oh545o2 and Oh51Ao2. | Zea mays |
yeast hom3/ura3 mutant sigmaa3hu was transformed with plasmids expressing the maize Ask2-Oh545o2 and Ask2-Oh51Ao2 sequences | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-lysine | Ask2-Oh545o2 and Ask2-Oh51Ao2 have similar lysine-sensitivity properties but differ in basal activity; Ask2-Oh545o2 and Ask2-Oh51Ao2 have similar lysine-sensitivity properties but differ in basal activity, to measure inhibition of AK activity by lysine, reaction rates using 50 mM Asp substrate are measured in the presence of 5 microM to 10 mM L-lysine | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | Zea mays | - |
ADP + 4-phospho-L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Zea mays | B0L9J2 | expression in Saccharomyces cerevisiae sigma a3hu | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Zea mays | ADP + 4-phospho-L-aspartate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ASK1 | - |
Zea mays |
ASK2 | - |
Zea mays |
aspartate kinase | - |
Zea mays |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.08 | - |
Ask2-Oh545o2 and Ask2-Oh51Ao2 | Zea mays | L-lysine | |
0.08 | - |
extract of transformed yeast hom3 cells by Ask2-Oh545o2 and Ask2-Oh51Ao2 | Zea mays | L-lysine |