Literature summary for 2.7.2.4 extracted from

Yoshida, A.; Tomita, T.; Kuzuyama, T.; Nishiyama, M.
Purification, crystallization and preliminary X-ray analysis of the regulatory subunit of aspartate kinase from Thermus thermophilus (2007), Acta Crystallogr. Sect. F, 63, 96-98.

Search for more literature for 2.7.2.4

Cloned(Commentary)

Cloned (Comment)	Organism
into the NdeI/HindIII site of pET26b(+) and introduced into Escherichia coli BL21-CodonPlus(DE3)-RIL cells	Thermus thermophilus
into vector pET26b(+) and introduced into Escherichia coli BL21-CodonPlus(DE3)-RIL cells	Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
by hanging-drop vapour-diffusion method, regulatory subunit in the presence of the inhibitor threonine, to 2.15 A resolution, crystal belongs to the cubic space group P4332 or P4132, with unit-cell parameters a = b = c = 141.8 A	Thermus thermophilus
by the hanging-drop vapour-diffusion method using Crystal Screen kits, the regulatory subunit (the beta subunit of Thermus thermophilus AK) is crystallized in the presence of the inhibitor threonine. Diffraction data are collected to 2.15 A at a synchrotron source. The crystal belongs to the cubic space group P4332 or P4132, with unit-cell parameters a = b = c = 141.8 A.	Thermus thermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
L-threonine	feedback inhibition by the end product	Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17720	-	full-length beta subunit (Met1Ala161) with a His6-tag extension at the C-terminal end, calculated	Thermus thermophilus
17720	-	beta subunit (Met1-Ala161), calculated from nucleotide sequence	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	P61489	-	-
Thermus thermophilus	P61489	expression in Escherichia coli BL21-CodonPlus(DE3)-RIL cells	-

Purification (Commentary)

Purification (Comment)	Organism
by nickel-affinity chromatography and gel filtration, to more than 95% homogeneity	Thermus thermophilus
nickel-affinity chromatography, gel-filtration FPLC column, more than 95% homogeneity of the purified subunit is verified by SDS-PAGE	Thermus thermophilus

Synonyms

Synonyms	Comment	Organism
AK	-	Thermus thermophilus
aspartate kinase	-	Thermus thermophilus

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Release 2023.1 (January 2023)